Search: onr:"swepub:oai:gup.ub.gu.se/336580" >
Combined effect of ...
Combined effect of salinity and hypoxia on digestive enzymes and intestinal microbiota in the oyster Crassostrea hongkongensis
-
Xie, Zhe (author)
-
Li, Yuting (author)
-
Xiong, Kai (author)
-
show more...
-
Tu, Zhihan (author)
-
Waiho, Khor (author)
-
Yang, Chuangye (author)
-
Deng, Yuewen (author)
-
Li, Saishuai (author)
-
K.H. Fang, James (author)
-
Hu, Menghong (author)
-
- Dupont, Samuel, 1971 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biologi och miljövetenskap, Kristineberg,Department of Biological and Environmental Sciences, Kristineberg
-
Wang, Youji (author)
-
show less...
-
(creator_code:org_t)
- 2023
- 2023
- English.
-
In: Environmental Pollution. - 0269-7491 .- 1873-6424. ; 331
- Related links:
-
https://gup.ub.gu.se...
-
show more...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Anthropologic activities caused frequent eutrophication in coastal and estuarine waters, resulting in diel-cycling hypoxia. Given global climate change, extreme weather events often occur, thus salinity fluctuation frequently breaks out in these waters. This study aimed to evaluate the combined effects of salinity and hypoxia on intestinal microbiota and digestive enzymes of Crassostrea hongkongensis. Specifically, we sequenced 16 S rRNA of intestinal microbiota and measured the digestive enzymes trypsin (TRS), lipase (LPS) and amylase (AMY) in oysters exposed for 28 days to three salinities (10, 25 and 35) and two dissolved oxygen conditions, normoxia (6 mg/L) and hypoxia (6 mg/L for 12 h, 2 mg/L for 12 h). Oysters in normoxia and salinity of 25 were treated as control. After 28-day exposure, for microbial components, Fusobacteriota, Firmicutes, Bacteroidota, Proteobacteria and Actinobacteriota comprised the majority for all experimental groups. Compared with the control group, the diversity and structure of intestinal microbiota tended to change in all treated groups. The species richness in C. hongkongensis intestine also changed. It was the most significant that high salinity increased Proteobacteria proportion while low salinity and hypoxia increased Fusobacteriota but decreased Proteobacteria, respectively. Additionally, Actinobacteriota was sensitive and changed under environmental stressor (P < 0.01). The prediction results on intestinal microbiota showed that, all functions of oysters were up-regulated to distinct degrees under low/high salinity with hypoxia. According to the KEGG prediction, cellular processes were more active and energy metabolism upregulated, indicating the adaptation of C. hongkongensis to environmental change. Periodical hypoxia and low/high salinity had complex effect on the digestive enzymes, in which the activity of TRS and LPS decreased while AMY increased. High/low salinity and periodical hypoxia can change the secretion of digestive enzymes and influence intestinal microbial diversity and species richness of C. hongkongensis, deducing the chronic adverse effects on the digestive physiology in long-term exposure.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- Digestion
- Hypoxia
- Intestinal microbiota
- Oyster
- Salinity
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Xie, Zhe
-
Li, Yuting
-
Xiong, Kai
-
Tu, Zhihan
-
Waiho, Khor
-
Yang, Chuangye
-
show more...
-
Deng, Yuewen
-
Li, Saishuai
-
K.H. Fang, James
-
Hu, Menghong
-
Dupont, Samuel, ...
-
Wang, Youji
-
show less...
- About the subject
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Biological Scien ...
- Articles in the publication
-
Environmental Po ...
- By the university
-
University of Gothenburg