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AdipoR2 recruits pr...
AdipoR2 recruits protein interactors to promote fatty acid elongation and membrane fluidity
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- Ruiz, Mario, 1984 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Devkota, Ranjan (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Kaper, Delaney (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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Ruhanen, Hanna (author)
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- Busayavalasa, Kiran (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Radovic, Uros, 1993 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Henricsson, Marcus, 1975 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Institute of Medicine, Department of Molecular and Clinical Medicine
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Käkelä, Reijo (author)
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- Borén, Jan, 1963 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Institute of Medicine, Department of Molecular and Clinical Medicine
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- Pilon, Marc, 1966 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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(creator_code:org_t)
- 2023
- 2023
- English.
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In: Journal of Biological Chemistry. - 0021-9258 .- 1083-351X. ; 299:6
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Abstract
Subject headings
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- The human AdipoR2 and its Caenorhabditis elegans homolog PAQR-2 are multipass plasma membrane proteins that protect cells against membrane rigidification. However, how AdipoR2 promotes membrane fluidity mechanistically is not clear. Using 13C-labeled fatty acids, we show that AdipoR2 can promote the elongation and incorporation of membrane-fluidizing polyunsaturated fatty acids into phospholipids. To elucidate the molecular basis of these activities, we performed immunoprecipitations of tagged AdipoR2 and PAQR-2 expressed in HEK293 cells or whole C. elegans, respectively, and identified coimmunoprecipitated proteins using mass spectrometry. We found that several of the evolutionarily conserved AdipoR2/PAQR-2 interactors are important for fatty acid elongation and incorporation into phospholipids. We experimentally verified some of these interactions, namely, with the dehydratase HACD3 that is essential for the third of four steps in long-chain fatty acid elongation and ACSL4 that is important for activation of unsaturated fatty acids and their channeling into phospholipids. We conclude that AdipoR2 and PAQR-2 can recruit protein interactors to promote the production and incorporation of unsaturated fatty acids into phospholipids.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- acyl-CoA synthetase
- adiponectin receptor
- Caenorhabditis elegans
- fatty acid
- HEK293
- immunoprecipitation
- Laurdan dye
- lipidomics
- membrane bilayer
- membrane fluidity
- membrane lipid
- PAQR
- phospholipid
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Ruiz, Mario, 198 ...
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Devkota, Ranjan
-
Kaper, Delaney
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Ruhanen, Hanna
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Busayavalasa, Ki ...
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Radovic, Uros, 1 ...
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show more...
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Henricsson, Marc ...
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Käkelä, Reijo
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Borén, Jan, 1963
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Pilon, Marc, 196 ...
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
- Articles in the publication
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Journal of Biolo ...
- By the university
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University of Gothenburg