RUNX transcription factors are essential in maintaining epididymal epithelial differentiation.

Apart from the androgen receptor, transcription factors (TFs) that are required for the development and formation of the different segments of the epididymis have remained unknown. We identified TF families expressed in the developing epididymides, of which many showed segment specificity. From these TFs, down-regulation of runt related transcription factors (RUNXs) 1 and 2 expression coincides with epithelial regression in Dicer1 cKO mice. Concomitant deletion of both Runx1 and Runx2 in a mouse epididymal epithelial cell line affected cell morphology, adhesion and mobility in vitro. Furthermore, lack of functional RUNXs severely disturbed the formation of 3D epididymal organoid-like structures. Transcriptomic analysis of the epididymal cell organoid-like structures indicated that RUNX1 and RUNX2 are involved in the regulation of MAPK signaling, NOTCH pathway activity, and EMT-related gene expression. This suggests that RUNXs are master regulators of several essential signaling pathways, and necessary for the maintenance of proper differentiation of the epididymal epithelium.

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Endokrinologi och diabetes hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Endocrinology and Diabetes hsv//eng
Humans
Male
Animals
Mice
Core Binding Factor Alpha 1 Subunit
genetics
Core Binding Factor Alpha 2 Subunit
genetics
Epididymis
Cell Differentiation
genetics
Cell Line

Björkgren, Ida (author)
Junnila, Arttu (author)
Mehmood, Arfa (author)
Mattsson, Jesse (author)
Raimoranta, Inka (author)
Kim, Bongki (author)
Laiho, Asta (author)
Nees, Matthias (author)
Elo, Laura (author)
Poutanen, MattiGothenburg University,Göteborgs universitet,Centre for Bone and Arthritis Research,Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition,Institute of Medicine, Department of Internal Medicine and Clinical Nutrition(Swepub:gu)xpouma (author)
Breton, Sylvie (author)
Sipilä, Petra (author)
Göteborgs universitetCentre for Bone and Arthritis Research (creator_code:org_t)

By the author/editor: Toriseva, Mervi; Björkgren, Ida; Junnila, Arttu; Mehmood, Arfa; Mattsson, Jesse; Raimoranta, Inka; show more...; Kim, Bongki; Laiho, Asta; Nees, Matthias; Elo, Laura; Poutanen, Matti; Breton, Sylvie; Sipilä, Petra; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Endocrinology an ...

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