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Experimental Evalua...
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Linnenbrink, Miriam
(author)
Experimental Evaluation of a Direct Fitness Effect of the De Novo Evolved Mouse Gene Pldi
- Article/chapterEnglish2024
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LIBRIS-ID:oai:gup.ub.gu.se/339068
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https://gup.ub.gu.se/publication/339068URI
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https://doi.org/10.1093/gbe/evae084DOI
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
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De novo evolved genes emerge from random parts of noncoding sequences and have, therefore, no homologs from which a function could be inferred. While expression analysis and knockout experiments can provide insights into the function, they do not directly test whether the gene is beneficial for its carrier. Here, we have used a seminatural environment experiment to test the fitness of the previously identified de novo evolved mouse gene Pldi, which has been implicated to have a role in sperm differentiation. We used a knockout mouse strain for this gene and competed it against its parental wildtype strain for several generations of free reproduction. We found that the knockout (ko) allele frequency decreased consistently across three replicates of the experiment. Using an approximate Bayesian computation framework that simulated the data under a demographic scenario mimicking the experiment's demography, we could estimate a selection coefficient ranging between 0.21 and 0.61 for the wildtype allele compared to the ko allele in males, under various models. This implies a relatively strong selective advantage, which would fix the new gene in less than hundred generations after its emergence.
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Breton, GwennaGothenburg University,Göteborgs universitet,Core Facilities, Genomics,Core Facilities, Genomics(Swepub:gu)xbregw
(author)
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Misra, Pallavi
(author)
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Pfeifle, Christine
(author)
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Dutheil, Julien Y.
(author)
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Tautz, Diethard
(author)
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Göteborgs universitetCore Facilities, Genomics
(creator_code:org_t)
Related titles
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In:GENOME BIOLOGY AND EVOLUTION16:51759-6653
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