Search: onr:"swepub:oai:gup.ub.gu.se/339685" >
Fecal microbiota tr...
Fecal microbiota transplantation of patients with anorexia nervosa did not alter flexible behavior in rats
-
Kooij, Karlijn L. (author)
-
Andreani, Nadia Andrea (author)
-
van der Gun, Luna L. (author)
-
show more...
-
Keller, Lara (author)
-
Trinh, Stefanie (author)
-
van der Vijgh, Benny (author)
-
Luijendijk, Mieneke (author)
-
Dempfle, Astrid (author)
-
Herpertz-Dahlmann, Beate (author)
-
Seitz, Jochen (author)
-
van Elburg, Annemarie (author)
-
Danner, Unna N. (author)
-
Baines, John (author)
-
- Adan, Roger A. H. (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi,Institute of Neuroscience and Physiology
-
show less...
-
(creator_code:org_t)
- 2024
- 2024
- English.
-
In: INTERNATIONAL JOURNAL OF EATING DISORDERS. - 0276-3478 .- 1098-108X.
- Related links:
-
https://gup.ub.gu.se...
-
show more...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- ObjectivePatients with anorexia nervosa (AN) are often anxious, display inflexible behavior and disrupted reward processing. Emerging evidence suggests that gut dysbiosis in patients contributes to the disease phenotype and progression.MethodsIn a preclinical study, we explored whether AN-derived microbiota impacts cognitive flexibility, anxiety, and dopamine signaling using fecal microbiota transplantation (FMT) in tyrosine hydroxylase-cre rats. We performed probabilistic reversal learning task (PRLT) at the baseline, after antibiotic treatment, and following FMT from patients with AN and controls. We assessed flexible behavior, task engagement, and ventral tegmental area (VTA) dopamine signaling during and in the absence of reward. Furthermore, anxiety-like behavior was evaluated with open field (OF) and elevated plus maze (EPM) tests.ResultsNeither antibiotic-induced dysbiosis nor AN FMT led to significant alterations in the number of reversals or lever press strategies after reinforced or nonreinforced lever presses (win and lose-stay) in the PRLT. However, the number of initiated trials decreased after antibiotic treatment while remaining unchanged after FMT. No significant differences were observed in VTA dopamine activity, anxiety measures in the OF and EPM tests. Microbiome analysis revealed limited overlap between the microbiota of the donors and recipients.DiscussionNo evidence was found that the microbiota of patients compared to controls, nor a depleted microbiome impacts cognitive flexibility. Nonetheless, antibiotic-induced dysbiosis resulted in reduced task engagement during the PRLT. The relatively low efficiency of the FMT is a limitation of our study and highlights the need for improved protocols to draw robust conclusions in future studies.Public SignificanceWhile our study did not reveal direct impacts of AN-associated gut microbiota on cognitive flexibility or anxiety behaviors in our preclinical model, we observed a decrease in task engagement after antibiotic-induced dysbiosis, underscoring that the presence of a gut microbiome matters. Our findings underscore the need for further refinement in FMT protocols to better elucidate the complex interplay between gut microbiota and behaviors characteristic of anorexia nervosa.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Neurologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Neurology (hsv//eng)
Keyword
- anorexia nervosa
- dopamine signaling
- fecal microbiota transplantation
- gut-brain axis
- reversal learning
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Kooij, Karlijn L ...
-
Andreani, Nadia ...
-
van der Gun, Lun ...
-
Keller, Lara
-
Trinh, Stefanie
-
van der Vijgh, B ...
-
show more...
-
Luijendijk, Mien ...
-
Dempfle, Astrid
-
Herpertz-Dahlman ...
-
Seitz, Jochen
-
van Elburg, Anne ...
-
Danner, Unna N.
-
Baines, John
-
Adan, Roger A. H ...
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Neurology
- Articles in the publication
-
INTERNATIONAL JO ...
- By the university
-
University of Gothenburg