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Heat shock protein-...
Heat shock protein-mediated resistance to high hydrostatic pressure in Escherichia coli
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Aertsen, A. (author)
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Vanoirbeek, K. (author)
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De Spiegeleer, P. (author)
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Sermon, J. (author)
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Hauben, K. (author)
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- Farewell, Anne, 1961 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi,Department of Cell and Molecular Biology
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- Nyström, Thomas, 1960 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi,Department of Cell and Molecular Biology
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Michiels, C. W. (author)
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(creator_code:org_t)
- 2004
- 2004
- English.
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In: Applied and Environmental Microbiology. - 0099-2240. ; 70:5, s. 2660-2666
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https://doi.org/10.1...
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Abstract
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- A random library of Escherichia coli MG1655 genomic fragments fused to a promoterless green fluorescent protein (GFP) gene was constructed and screened by differential fluorescence induction for promoters that are induced after exposure to a sublethal high hydrostatic pressure stress. This screening yielded three promoters of genes belonging to the heat shock regulon (dnaK, lon, clpPX), suggesting a role for heat shock proteins in protection against, and/or repair of, damage caused by high pressure. Several further observations provide additional support for this hypothesis: (i) the expression of rpoH, encoding the heat shock-specific sigma factor {sigma}32, was also induced by high pressure; (ii) heat shock rendered E. coli significantly more resistant to subsequent high-pressure inactivation, and this heat shock-induced pressure resistance followed the same time course as the induction of heat shock genes; (iii) basal expression levels of GFP from heat shock promoters, and expression of several heat shock proteins as determined by two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis of proteins extracted from pulse-labeled cells, was increased in three previously isolated pressure-resistant mutants of E. coli compared to wild-type levels.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Aertsen, A.
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Vanoirbeek, K.
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De Spiegeleer, P ...
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Sermon, J.
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Hauben, K.
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Farewell, Anne, ...
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show more...
-
Nyström, Thomas, ...
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Michiels, C. W.
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
- Articles in the publication
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Applied and Envi ...
- By the university
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University of Gothenburg