onr:"swepub:oai:gup.ub.gu.se/41523"
Search: onr:"swepub:oai:gup.ub.gu.se/41523" > The effects of endo...
- 1 of 1
- Previous record
- Next record
- To hitlist
- Holmberg, Anna,1974Gothenburg University,Göteborgs universitet,Zoologiska institutionen,Department of Zoology (author)
The effects of endogenous and exogenous nitric oxide on gut motility in zebrafish Danio rerio embryos and larvae
- Article/chapterEnglish2006
Publisher, publication year, extent ...
- The Company of Biologists,2006
Numbers
- LIBRIS-ID:oai:gup.ub.gu.se/41523
- https://gup.ub.gu.se/publication/41523URI
- https://doi.org/10.1242/jeb.02272DOI
Supplementary language notes
- Language:English
Part of subdatabase
- SwePubSwePub
Classification
Notes
- Using motion analysis, the ontogeny of the nitrergic control system in the gut was studied in vivo in zebrafish Danio rerio embryos and larvae. For the first time we show the presence of a nitrergic tonus, modulating both anterograde and retrograde contraction waves in the intestine of developing zebrafish. At 4 d.p.f. (days post fertilisation), the nitric oxide synthase (NOS) inhibitor L-NAME (three boluses of 50-100 nl, 10(-3) mol l(-1)) increased the anterograde contraction wave frequency by 0.50 +/- 0.10 cycles min(-1). Subsequent application of the NO donor sodium nitroprusside (SNP; three boluses of 50-100 nl, 10(-4) mol l(-1)) reduced the frequency of propagating anterograde waves (-0.71 +/- 0.20 cycles min(-1)). This coincided with the first appearance of an excitatory cholinergic tonus, observed in an earlier study. One day later, at 5 d.p.f., in addition to the effect on anterograde contraction waves, application of L-NAME increased (0.39 +/- 0.15 cycles min(-1)) and following SNP application reduced (-1.61 +/- 0.36 cycles min(-1)) the retrograde contraction wave frequency. In contrast, at 3 d.p.f., when no spontaneous motility is observed, application of L-NAME did not induce contraction waves in either part of the gut, indicating the lack of a functional inhibitory tonus at this early stage. Gut neurons expressing NOS-like immunoreactivity were present in the distal and middle intestine as early as 2 d.p.f., and at 1 day later in the proximal intestine. In conclusion, the present study suggests that a nitrergic inhibitory tonus develops shortly before or at the time for onset of exogenous feeding.
Subject headings and genre
- NATURVETENSKAP Biologi hsv//swe
- NATURAL SCIENCES Biological Sciences hsv//eng
- enteric nervous system
- l-name
- sodium nitroprusside
- ontogeny
- zebrafish danio rerio
- enteric nervous-system
- vasoactive intestinal polypeptide
- nadph-diaphorase activity
- gadus-morhua
- atlantic cod
- chicken gut
- immunohistochemical analysis
- gastrointestinal motility
- ambystoma-mexicanum
- interstitial-cells
Added entries (persons, corporate bodies, meetings, titles ...)
- Olsson, Catharina,1968Gothenburg University,Göteborgs universitet,Zoologiska institutionen,Department of Zoology(Swepub:gu)xolcat (author)
- Holmgren, Susanne,1946Gothenburg University,Göteborgs universitet,Zoologiska institutionen,Department of Zoology(Swepub:gu)xholsu (author)
- Göteborgs universitetZoologiska institutionen (creator_code:org_t)
Related titles
- In:Journal of Experimental Biology: The Company of Biologists209:13, s. 2472-24790022-09491477-9145
Internet link
Find in a library
- Journal of Experimental Biology (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Holmberg, Anna, ...
- Olsson, Catharin ...
- Holmgren, Susann ...
- About the subject
-
- NATURAL SCIENCES
- NATURAL SCIENCES
- and Biological Scien ...
- Articles in the publication
- Journal of Exper ...
- By the university
- University of Gothenburg
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
