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Selective introduct...
Selective introduction of antisense oligonucleotides into single adult CNS progenitor cells using electroporation demonstrates the requirement of STAT3 activation for CNTF-induced gliogenesis
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- Åberg, Maria A I, 1972 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för klinisk neurovetenskap, Sektionen för laborativ neurovetenskap,Institute of Clinical Neurosciences, Section of Experimental Neuroscience
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- Ryttsén, Frida (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry
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- Hellgren, Gunnel, 1961 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kvinnors och barns hälsa, Avdelningen för pediatrik,Institute for the Health of Women and Children, Dept of Paediatrics
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- Lindell, Kajsa, 1971 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för kroppssammansättning och metabolism,Institute of Internal Medicine, Dept of Body Composition and Metabolism
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- Rosengren, Lars, 1954 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för klinisk neurovetenskap,Institute of Clinical Neurosciences
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MacLennan, A. J. (author)
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- Carlsson, Björn, 1958 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för kroppssammansättning och metabolism,Institute of Internal Medicine, Dept of Body Composition and Metabolism
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- Orwar, Owe (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry
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- Eriksson, Peter S, 1959 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för klinisk neurovetenskap,Institute of Clinical Neurosciences
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(creator_code:org_t)
- Elsevier BV, 2001
- 2001
- English.
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In: Mol Cell Neurosci. - : Elsevier BV. ; 17:3, s. 426-43
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Abstract
Subject headings
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- We have developed a novel method in which antisense DNA is selectively electroporated into individual adult neural progenitor cells. By electroporation of antisense oligonucleotides against signal transducer and activator of transcription 3 (STAT3) we demonstrate that ciliary neurotrophic factor (CNTF) is an instructive signal for astroglial type 2 cell fate specifically mediated via activation of STAT3. Activation of the mitogen-activated protein kinase (MAPK) signaling pathway induced only a transient increase in glial fibrillary acidic protein (GFAP) expression, and inhibition of this signaling pathway did not block the induction by CNTF of glial differentiation in progenitor cells. In addition we show that microelectroporation is a new powerful method for introducing antisense agents into single cells in complex cellular networks.
Keyword
- Age Factors
- Animals
- Astrocytes/*cytology/enzymology
- Cell Differentiation/drug effects/physiology
- Cell Division/drug effects/physiology
- Cells
- Cultured
- Ciliary Neurotrophic Factor/*pharmacology
- DNA-Binding Proteins/*genetics/metabolism
- Electroporation
- Enzyme Inhibitors/pharmacology
- Flavonoids/pharmacology
- Gene Expression/physiology
- Genistein/pharmacology
- Glial Fibrillary Acidic Protein/metabolism
- Hippocampus/*cytology
- MAP Kinase Signaling System/physiology
- Oligonucleotides
- Antisense/*pharmacology
- Protein-Tyrosine Kinase/metabolism
- Rats
- Stem Cells/cytology/*enzymology
- Support
- Non-U.S. Gov't
- Trans-Activators/*genetics/metabolism
- Transfection
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Åberg, Maria A I ...
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Ryttsén, Frida
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Hellgren, Gunnel ...
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Lindell, Kajsa, ...
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Rosengren, Lars, ...
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MacLennan, A. J.
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Carlsson, Björn, ...
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Orwar, Owe
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Eriksson, Peter ...
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- Articles in the publication
- Mol Cell Neurosc ...
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Molecular and Ce ...
- By the university
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University of Gothenburg