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Influence of temper...
Influence of temperature on radiation-induced inhibition of DNA supercoiling
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- Elmroth, Kerstin, 1970 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för särskilda specialiteter, Avdelningen för onkologi,Institute of Selected Clinical Sciences, Department of Oncology
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Erkell, L. J. (author)
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- Hultborn, Ragnar, 1946 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för särskilda specialiteter, Avdelningen för onkologi,Institute of Selected Clinical Sciences, Department of Oncology
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(creator_code:org_t)
- 1999
- 1999
- English.
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In: Radiat Res. - 0033-7587. ; 152:2, s. 137-43
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https://gup.ub.gu.se...
Abstract
Subject headings
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- The influence of subnormal temperatures (2, 15 and 28 degrees C) on the effects of radiation in MCF-7 cell cultures was studied using the fluorescent (halo) nucleoid assay. Increasing the propidium iodide (PI) concentration (0.5-7.5 microgram/ml PI) resulted in relaxation, i.e. in increasing nucleoid area; higher concentrations up to 50 microgram/ml caused rewinding that resulted in nucleoid contraction. Rewinding was inhibited by X irradiation (2, 4 and 8 Gy) in a dose-dependent way. Incubation at subnormal temperature did not influence the nucleoid area but did reduce radiation-induced inhibition of rewinding after 4 Gy. The low temperature (2 degrees C) during rather than prior to irradiation appeared to protect from radiation-induced inhibition of nucleoid rewinding. Decreased temperature during irradiation may change the conditions so as to reduce DNA- matrix damage induced by radiation.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Keyword
- *DNA Damage
- DNA
- Superhelical/*radiation effects
- Dose-Response Relationship
- Radiation
- Humans
- Propidium
- *Temperature
- Tumor Cells
- Cultured
Publication and Content Type
- ref (subject category)
- art (subject category)
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