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Influence of temper...
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Elmroth, Kerstin,1970Gothenburg University,Göteborgs universitet,Institutionen för särskilda specialiteter, Avdelningen för onkologi,Institute of Selected Clinical Sciences, Department of Oncology
(author)
Influence of temperature on radiation-induced inhibition of DNA supercoiling
- Article/chapterEnglish1999
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LIBRIS-ID:oai:gup.ub.gu.se/45513
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https://gup.ub.gu.se/publication/45513URI
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
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The influence of subnormal temperatures (2, 15 and 28 degrees C) on the effects of radiation in MCF-7 cell cultures was studied using the fluorescent (halo) nucleoid assay. Increasing the propidium iodide (PI) concentration (0.5-7.5 microgram/ml PI) resulted in relaxation, i.e. in increasing nucleoid area; higher concentrations up to 50 microgram/ml caused rewinding that resulted in nucleoid contraction. Rewinding was inhibited by X irradiation (2, 4 and 8 Gy) in a dose-dependent way. Incubation at subnormal temperature did not influence the nucleoid area but did reduce radiation-induced inhibition of rewinding after 4 Gy. The low temperature (2 degrees C) during rather than prior to irradiation appeared to protect from radiation-induced inhibition of nucleoid rewinding. Decreased temperature during irradiation may change the conditions so as to reduce DNA- matrix damage induced by radiation.
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Erkell, L. J.
(author)
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Hultborn, Ragnar,1946Gothenburg University,Göteborgs universitet,Institutionen för särskilda specialiteter, Avdelningen för onkologi,Institute of Selected Clinical Sciences, Department of Oncology(Swepub:gu)xhulra
(author)
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Göteborgs universitetInstitutionen för särskilda specialiteter, Avdelningen för onkologi
(creator_code:org_t)
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In:Radiat Res152:2, s. 137-430033-7587
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Radiat Res
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