A new combined multicompartmental model for apolipoprotein B-100 and triglyceride metabolism in VLDL subfractions

Adiels, Martin,1976Gothenburg University,Göteborgs universitet,Institutionen för matematiska vetenskaper,Department of Mathematical Sciences,University of Gothenburg,Chalmers tekniska högskola,Chalmers University of Technology (author)

The use of stable isotopes in conjunction with compartmental modeling analysis has greatly facilitated studies of the metabolism of the apolipoprotein B (apoB)-containing lipoproteins in humans. The aim of this study was to develop a multicompartment model that allows us to simultaneously determine the kinetics of apoB and triglyceride (TG) in VLDL(1) and VLDL(2) after a bolus injection of [(2)H(3)]leucine and [(2)H(5)]glycerol and to follow the catabolism and transfer of the lipoprotein particles. Here, we describe the model and present the results of its application in a fasting steady-state situation in 17 subjects with lipid values representative of a Western population. Analysis of the correlations showed that plasma TG was determined by the VLDL(1) and VLDL(2) apoB and TG fractional catabolic rate. Furthermore, the model showed a linear correlation between VLDL(1) TG and apoB production. A novel observation was that VLDL TG entered the circulation within 21 min after its synthesis, whereas VLDL apoB entered the circulation after 33 min. These observations are consistent with a sequential assembly model of VLDL and suggest that the TG is added to a primordial apoB-containing particle in the liver.

Packard, C. (author)
Caslake, M. J. (author)
Stewart, P. (author)
Soro, A. (author)
Westerbacka, J. (author)
Wennberg, Bernt,1961Gothenburg University,Göteborgs universitet,Institutionen för matematiska vetenskaper,Department of Mathematical Sciences,Chalmers tekniska högskola,Chalmers University of Technology,University of Gothenburg(Swepub:cth)wennberg (author)
Olofsson, Sven-Olof,1947Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Wallenberg Laboratory,University of Gothenburg(Swepub:gu)xolosv (author)
Taskinen, M. R. (author)
Borén, Jan,1963Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Wallenberg Laboratory,University of Gothenburg(Swepub:gu)xborej (author)
Göteborgs universitetInstitutionen för matematiska vetenskaper (creator_code:org_t)

By the author/editor: Adiels, Martin, ...; Packard, C.; Caslake, M. J.; Stewart, P.; Soro, A.; Westerbacka, J.; show more...; Wennberg, Bernt, ...; Olofsson, Sven-O ...; Taskinen, M. R.; Borén, Jan, 1963; show less...

By the university: University of Gothenburg; Chalmers University of Technology

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