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Large-scale identif...
Large-scale identification of genes implicated in kidney glomerulus development and function.
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- Takemoto, Minoru (author)
- Karolinska Institutet
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- He, Liqun (author)
- Karolinska Institutet
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- Norlin, Jenny (author)
- Karolinska Institutet
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- Patrakka, Jaakko (author)
- Karolinska Institutet
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- Xiao, Zhijie (author)
- Karolinska Institutet
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- Petrova, Tatiana (author)
- Helsingin Yliopisto,University of Helsinki
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- Bondjers, Cecilia, 1974 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology,University of Gothenburg
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- Asp, Julia, 1973 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för patologi,Institute of Biomedicine, Department of Pathology,University of Gothenburg
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- Wallgard, Elisabet (author)
- Karolinska Institutet
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- Sun, Ying, 1955 (author)
- Karolinska Institutet
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- Samuelsson, Tore, 1951 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology,University of Gothenburg
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- Mostad, Petter, 1964 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för matematiska vetenskaper, matematisk statistik,Department of Mathematical Sciences, Mathematical Statistics,Chalmers tekniska högskola,Chalmers University of Technology,University of Gothenburg
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- Lundin, Samuel B, 1970 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology,University of Gothenburg
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- Miura, Naoyuki (author)
- Hamamatsu University
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- Sado, Yoshikazu (author)
- Shigei Medical Research Institute
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- Alitalo, Kari (author)
- Helsingin Yliopisto,University of Helsinki
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- Quaggin, Susan E (author)
- University of Toronto
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- Tryggvason, Karl (author)
- Karolinska Institutet
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- Betsholtz, Christer, 1959 (author)
- Karolinska Institutet
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(creator_code:org_t)
- 2006-02-23
- 2006
- English.
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In: The EMBO journal. - : Wiley. - 0261-4189 .- 1460-2075. ; 25:5, s. 1160-74
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Abstract
Subject headings
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- To advance our understanding of development, function and diseases in the kidney glomerulus, we have established and large-scale sequenced cDNA libraries from mouse glomeruli at different stages of development, resulting in a catalogue of 6053 different genes. The glomerular cDNA clones were arrayed and hybridized against a series of labeled targets from isolated glomeruli, non-glomerular kidney tissue, FACS-sorted podocytes and brain capillaries, which identified over 300 glomerular cell-enriched transcripts, some of which were further sublocalized to podocytes, mesangial cells and juxtaglomerular cells by in situ hybridization. For the earliest podocyte marker identified, Foxc2, knockout mice were used to analyze the role of this protein during glomerular development. We show that Foxc2 controls the expression of a distinct set of podocyte genes involved in podocyte differentiation and glomerular basement membrane maturation. The primary podocyte defects also cause abnormal differentiation and organization of the glomerular vascular cells. We surmise that studies on the other novel glomerulus-enriched transcripts identified in this study will provide new insight into glomerular development and pathomechanisms of disease.
Keyword
- Animals
- Basement Membrane
- cytology
- metabolism
- Brain
- cytology
- metabolism
- Cell Differentiation
- Female
- Forkhead Transcription Factors
- genetics
- physiology
- Gene Expression Profiling
- Gene Expression Regulation
- Developmental
- Gene Library
- Glomerular Mesangium
- cytology
- metabolism
- In Situ Hybridization
- Integrases
- metabolism
- Kidney Glomerulus
- cytology
- physiology
- Male
- Mice
- Mice
- Inbred ICR
- Mice
- Knockout
- Nephrons
- cytology
- metabolism
- Oligonucleotide Array Sequence Analysis
- Podocytes
- cytology
- metabolism
- metabolism
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
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Takemoto, Minoru
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He, Liqun
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Norlin, Jenny
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Patrakka, Jaakko
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Xiao, Zhijie
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Petrova, Tatiana
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show more...
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Bondjers, Cecili ...
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Asp, Julia, 1973
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Wallgard, Elisab ...
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Sun, Ying, 1955
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Samuelsson, Tore ...
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Mostad, Petter, ...
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Lundin, Samuel B ...
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Miura, Naoyuki
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Sado, Yoshikazu
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Alitalo, Kari
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Quaggin, Susan E
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Tryggvason, Karl
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Betsholtz, Chris ...
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show less...
- Articles in the publication
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The EMBO journal
- By the university
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University of Gothenburg
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Chalmers University of Technology
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Karolinska Institutet