Search: onr:"swepub:oai:gup.ub.gu.se/52270" >
The immunohistochem...
The immunohistochemical expression of DNA-PKCS and Ku (p70/p80) in head and neck cancers: relationships with radiosensitivity
-
- Björk-Eriksson, Thomas, 1960 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för särskilda specialiteter, Avdelningen för onkologi,Institute of Selected Clinical Sciences, Department of Oncology
-
West, C. (author)
-
- Nilsson, A. (author)
- Karolinska Institutet
-
show more...
-
Magnusson, B. (author)
-
Svensson, M. (author)
-
Karlsson, E. (author)
-
Slevin, N. (author)
-
- Lewensohn, R. (author)
- Karolinska Institutet
-
- Mercke, Claes, 1941 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för särskilda specialiteter, Avdelningen för onkologi,Institute of Selected Clinical Sciences, Department of Oncology
-
show less...
-
(creator_code:org_t)
- 1999
- 1999
- English.
-
In: Int J Radiat Oncol Biol Phys. - 0360-3016. ; 45:4, s. 1005-10
- Related links:
-
https://gup.ub.gu.se...
-
show more...
-
http://kipublication...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- PURPOSE: The DNA-PK complex is one of the major pathways by which mammalian cells respond to DNA double-strand breaks induced by ionizing radiation. This study evaluated the relationship between the immunohistochemical expression of the individual components of DNA-PK and cellular radiosensitivity in head and neck cancers. METHODS AND MATERIALS: Biopsies from patients with previously untreated squamous cell carcinomas of the head and neck were assessed for inherent tumor radiosensitivity measured as the surviving fraction at 2 Gy (SF2) using a soft agar clonogenic assay. Paraffin-embedded tumor material from 64 successfully grown specimens was immunohistochemically stained for expression of DNA-PKcs and Ku (p70/p80). The same tumor material was previously analyzed for the immunohistochemical expression of p53. RESULTS: A significant correlation was found between the degree of expression of DNA-PKcs and Ku (p70/p80) (r = 0.55, p<0.001). There were no overall significant differences in the levels of expression of DNA-PKcs and Ku (p70/p80) in tumors from patients of either sex, different sites, histologies, and stages. No relationship was found between SF2 and the expression of either DNA-PKcs (r = 0.22, p = 0.081) or Ku (p70/p80) (r = 0.064, p = 0.62). Comparison with previous immunohistochemical characterization showed no significant correlations between the expression levels of p53 and either DNA-PKcs (r = 0.093, p = 0.46) or Ku (p70/p80) (r = -0.17, p = 0.17). CONCLUSIONS: This study suggests that determining the immunohistochemical expression of DNA-PK in head and neck cancers from multiple sites does not have a role as a predictive assay of tumor in vitro radiosensitivity.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Keyword
- *Antigens
- Nuclear
- Calcium-Binding Proteins/*metabolism
- *DNA Helicases
- DNA
- Neoplasm/*radiation effects
- DNA-Binding Proteins/*metabolism
- Female
- Head and Neck Neoplasms/genetics/*metabolism/radiotherapy
- Humans
- Immunohistochemistry
- Male
- Neoplasm Proteins/*metabolism
- Nuclear Proteins/*metabolism
- Radiation Tolerance
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Björk-Eriksson, ...
-
West, C.
-
Nilsson, A.
-
Magnusson, B.
-
Svensson, M.
-
Karlsson, E.
-
show more...
-
Slevin, N.
-
Lewensohn, R.
-
Mercke, Claes, 1 ...
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Cancer and Oncol ...
- Articles in the publication
-
Int J Radiat Onc ...
- By the university
-
University of Gothenburg
-
Karolinska Institutet