Detection of antibodies to toxin-coregulated pili in sera from cholera patients

• Monoclonal antibodies (MAbs) were prepared against toxin-coregulated pili (TCP) isolated from Vibrio cholerae O1 El Tor. Despite their limited bactericidal potential, two MAbs were able to mediate biotype-specific protection against experimental cholera in infant mice. These MAbs were used in immunoblotting studies to assess seroconversion to El Tor TCP following cholera. Clear anti-pilus responses were observed in five of nine patients.

Keyword

Animals

Antibodies

Bacterial/administration & dosage/*blood

Antibodies

Monoclonal/administration & dosage

Antibody Specificity

Antigens

Bacterial

Bacterial Toxins/antagonists & inhibitors/*immunology

Cholera/*immunology/microbiology/prevention & control

Cholera Vaccines/administration & dosage

Fimbriae

Bacterial/*immunology

Humans

Mice

Vibrio cholerae O1/*immunology

Publication and Content Type

ref (subject category)

art (subject category)