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Assessment of allel...
Assessment of allele dosage at polymorphic microsatellite loci displaying allelic imbalance in tumors by means of quantitative competitive-polymerase chain reaction.
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- Sjöling, Åsa, 1968 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicinsk mikrobiologi och immunologi,Institutionen för cell- och molekylärbiologi,Institute of Medical Microbiology/Immunology,Department of Cell and Molecular Biology,Department of Cell and Molecular Biology-Genetics, Göteborg, Sweden ; Department of Medical Microbiology and Immunology, Göteborg, Sweden
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- Walentinsson, Anna, 1971 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi,Department of Cell and Molecular Biology,Department of Cell and Molecular Biology-Genetics, Göteborg, Sweden
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- Nordlander, Carola, 1969 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi,Department of Cell and Molecular Biology,Department of Cell and Molecular Biology-Genetics, Göteborg, Sweden
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- Karlsson, Åsa (author)
- Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi,Department of Cell and Molecular Biology,Department of Cell and Molecular Biology-Genetics, Göteborg, Sweden
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- Behboudi, Afrouz, 1967 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi,Department of Cell and Molecular Biology,Department of Cell and Molecular Biology-Genetics, Göteborg, Sweden
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- Samuelson, Emma, 1974 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi,Department of Cell and Molecular Biology,Department of Cell and Molecular Biology-Genetics, Göteborg, Sweden
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- Levan, Göran, 1939 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi,Department of Cell and Molecular Biology,Department of Cell and Molecular Biology-Genetics, Göteborg, Sweden
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- Röhme, Dan, 1944 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi,Department of Cell and Molecular Biology,Department of Cell and Molecular Biology-Genetics, Göteborg, Sweden
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(creator_code:org_t)
- Elsevier BV, 2005
- 2005
- English.
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In: Cancer genetics and cytogenetics. - : Elsevier BV. - 0165-4608 .- 1873-4456. ; 157:2, s. 97-103
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Abstract
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- Analysis of allelic imbalance at polymorphic marker loci is usually employed to identify chromosomal regions affected by recurrent aberrations in tumor genomes. Such regions are likely to harbor genes involved in the onset and/or progression of cancer. Although often used to identify regions of loss of heterozygosity caused by deletions/rearrangements near tumor suppressor gene loci, allelic imbalance can also reflect regional amplification, indicating the presence of oncogenes. It is difficult to tell these two situations apart after ordinary polymerase chain reaction (PCR), but here we describe a method that distinguishes allelic loss from allelic gain. The level of allelic imbalance was determined by quantitative PCR (QPCR) in the presence of an internal control DNA that displayed a third allele at the locus studied. To validate the efficiency of allele quantitation, we analyzed an amplified region in a set of rat fibrosarcomas. In four tumor samples with amplification of the Met oncogene, we could show with QPCR that there was amplification of one of the alleles at a microsatellite marker located close to Met. QPCR may be useful for cancer studies because experiments may be predesigned for using either suitable microsatellite markers or the abundant and polymorphic poly-A tails of rodent identifier sequences.
Subject headings
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Medicinsk genetik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medical Genetics (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Keyword
- Allelic Imbalance
- Animals
- Fibrosarcoma
- genetics
- Gene Dosage
- Microsatellite Repeats
- Polymerase Chain Reaction
- methods
- Polymorphism
- Restriction Fragment Length
- Rats
- Rats
- Inbred Strains
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Sjöling, Åsa, 19 ...
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Walentinsson, An ...
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Nordlander, Caro ...
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Karlsson, Åsa
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Behboudi, Afrouz ...
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Samuelson, Emma, ...
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show more...
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Levan, Göran, 19 ...
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Röhme, Dan, 1944
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Genetics
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Medical Genetics
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Cancer and Oncol ...
- Articles in the publication
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Cancer genetics ...
- By the university
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University of Gothenburg
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University of Skövde