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Intestinal alpha be...
Intestinal alpha beta T cells differentiate and rearrange antigen receptor genes in situ in the human infant.
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Williams, Amanda M (author)
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- Bland, Paul William, 1949 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin, Avdelningen för klinisk immunologi,Institute of Laboratory Medicine, Dept of Clinical Immunology
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Phillips, Anne C (author)
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Turner, Susan (author)
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Brooklyn, Trevor (author)
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Shaya, Gabriel (author)
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Spicer, Richard D (author)
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Probert, Christopher S J (author)
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(creator_code:org_t)
- 2004
- 2004
- English.
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In: Journal of immunology (Baltimore, Md. : 1950). - 0022-1767. ; 173:12, s. 7190-9
- Related links:
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https://gup.ub.gu.se...
Abstract
Subject headings
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- Intestinal Ag exposure during neonatal life influences appropriate adult immune responses. To define the mechanisms shaping the T cell repertoire during this period, we examined T cell differentiation and receptor diversity in the intestine of human infants. Developmental phenotypes of intraepithelial and lamina propria intestinal T cells from infants aged 1 day to 2 years were assessed ex vivo by flow cytometry and in situ by triple-fluorescent immunohistochemistry. Gene recombination-specific enzymes were assessed by PCR. TCR beta-chain V region gene diversity was determined by sequencing. Several different early lineage T cell populations were present neonatally: CD3(+)4(-)8(-) T cells were present at birth and numbers decreased during the neonatal period; CD3(+)4(+)8(+) T cells were present in low numbers throughout infancy; and CD3(+)4(+)8(-) or CD3(+)4(-)8(+) T cells increased with age. Very early lineage T cells, CD3(-)2(-)7(+) and CD3(-)2(+)7(+), were present neonatally, but were essentially absent at 1 year. Most lamina propria T cells differentiated rapidly after birth, but maturation of intraepithelial T cells took place over 1 year. Intestinal samples from infants less than 6 mo old contained transcripts of T early alpha and TdT, and 15 of 19 infant samples contained mRNA for RAG-1, some coexpressing RAG-2. TCR beta-chain repertoires were polyclonal in infants. Immature T cells, pre-T cells, and genes involved in T cell recombination were found in the intestine during infancy. T cell differentiation occurs within the neonatal human intestine, and the TCR repertoire of these developing immature T cells is likely to be influenced by luminal Ags. Thus, mucosal T cell responsiveness to environmental Ag is shaped in situ during early life.
Keyword
- Adolescent
- Aging
- genetics
- immunology
- Cell Differentiation
- genetics
- immunology
- Child
- Preschool
- Clone Cells
- Gene Rearrangement
- alpha-Chain T-Cell Antigen Receptor
- Gene Rearrangement
- beta-Chain T-Cell Antigen Receptor
- Humans
- Immunophenotyping
- Infant
- Infant
- Newborn
- Intestinal Mucosa
- cytology
- immunology
- metabolism
- Intestine
- Large
- cytology
- immunology
- metabolism
- Intestine
- Small
- cytology
- immunology
- metabolism
- Lymphocyte Count
- Organ Specificity
- genetics
- immunology
- Receptors
- Antigen
- T-Cell
- alpha-beta
- biosynthesis
- genetics
- Recombination
- Genetic
- Stem Cells
- cytology
- immunology
- metabolism
- T-Lymphocyte Subsets
- cytology
- immunology
- metabolism
Publication and Content Type
- ref (subject category)
- art (subject category)
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