Search: onr:"swepub:oai:gup.ub.gu.se/54794" >
Fatty acids induce ...
Fatty acids induce increased granulocyte macrophage-colony stimulating factor secretion through protein kinase C-activation in THP-1 macrophages.
-
Bahramian, Nahid (author)
-
- Östergren Lundén, Gunnel, 1950 (author)
- Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Wallenberg Laboratory
-
- Bondjers, Göran, 1944 (author)
- Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Wallenberg Laboratory
-
show more...
-
Olsson, Urban (author)
-
show less...
-
(creator_code:org_t)
- 2004
- 2004
- English.
-
In: Lipids. - 0024-4201. ; 39:3, s. 243-9
- Related links:
-
https://gup.ub.gu.se...
Abstract
Subject headings
Close
- Insulin resistance and type 2 diabetes are associated with elevated circulating levels of nonesterified FA (NEFA) and lipoprotein remnants. The dyslipidemia is an important contributor to the excess arterial disease associated with insulin resistance and type 2 diabetes, but the mechanisms involved are elusive. In the present study we examined the effect of NEFA on macrophages. For this purpose, we utilized human macrophages, prepared by treating THP-1 monocytes with phorbol ester. We found that albumin-bound NEFA at physiological levels increase the secretion of granulocyte macrophage-colony stimulating factor (GM-CSF) by the THP-1 macrophages in a dose-dependent manner. The effect was registered as an increase in mRNA, and the amount of GM-CSF secreted correlated with the accumulation of TAG and DAG in the cell. The NEFA-induced rise in GM-CSF appeared to be mediated by activation of protein kinase C, probably acting on extracellular signal-regulated kinases 1 and 2 and being calcium dependent. We speculate that increased secretion of GM-CSF by resident macrophages in the intima exposed chronically to high levels of NEFA, such as those present in insulin resistance, may contribute to a proatherogenic response of arterial cells.
Keyword
- Calcium Channel Blockers
- pharmacology
- Cell Line
- Enzyme Activation
- Fatty Acids
- pharmacology
- Flavonoids
- pharmacology
- Granulocyte-Macrophage Colony-Stimulating Factor
- genetics
- metabolism
- Humans
- Linoleic Acid
- pharmacology
- Macrophages
- drug effects
- enzymology
- Mitogen-Activated Protein Kinase 3
- metabolism
- Nifedipine
- pharmacology
- Phosphorylation
- drug effects
- Protein Kinase C
- metabolism
- Protein Kinase Inhibitors
- pharmacology
- RNA
- Messenger
- analysis
- Tetradecanoylphorbol Acetate
- pharmacology
- Up-Regulation
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
-
Lipids
(Search for host publication in LIBRIS)
To the university's database