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Genotyping of clinical herpes simplex virus type 1 isolates by use of restriction enzymes.
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- Norberg, Peter, 1974 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine
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- Bergström, Tomas, 1950 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine
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- Liljeqvist, Jan-Åke, 1954 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine
- (creator_code:org_t)
- 2006
- 2006
- English.
- In: Journal of clinical microbiology. - 0095-1137. ; 44:12, s. 4511-4
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Recently, three distinct genotypes of clinical herpes simplex virus type 1 (HSV-1) isolates were identified based on DNA sequence information and phylogenetic analysis of clinical isolates and laboratory strains. We utilized single-nucleotide polymorphism within the genes coding for glycoproteins G and I for rapid genotype classification by PCR and restriction enzyme cleavage. The method is suitable for high-scale genotyping of clinical HSV-1 isolates and for the detection of recombinants.
Keyword
- Cluster Analysis
- DNA Fingerprinting
- methods
- DNA Restriction Enzymes
- DNA
- Viral
- genetics
- Genotype
- Herpes Simplex
- virology
- Herpesvirus 1
- Human
- classification
- genetics
- Humans
- Polymerase Chain Reaction
- Polymorphism
- Restriction Fragment Length
- Polymorphism
- Single Nucleotide
- Viral Envelope Proteins
- genetics
Publication and Content Type
- ref (subject category)
- art (subject category)
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