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Chromosomal integri...
Chromosomal integrity maintained in five human embryonic stem cell lines after prolonged in vitro culture
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Caisander, Gunilla (author)
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- Park, Hanna (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences
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Frej, K. (author)
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Lindqvist, J. (author)
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- Bergh, Christina, 1953 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences
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- Lundin, Kersti, 1957 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences
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- Hanson, Charles, 1958 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences
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(creator_code:org_t)
- 2006
- 2006
- English.
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In: Chromosome Res. ; 14:2, s. 131-7
- Related links:
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Abstract
Subject headings
Close
- There have been recent reports of human embryonic stem cell (hESC) lines developing chromosomal aberrations after long-term culture, indicating an unstable genomic status due to the in vitro milieu. This raises concern, since it would limit their use in therapeutics. In this study the chromosomal status of five well-characterized hESC lines, SA002, SA002.5, AS034.1.1, SA121 and SA461, was monitored during long-term in vitro culture. The criteria of defined hESCs were met by all of the five hESC lines (four diploid and one trisomic for chromosome 13). The genomes were screened for chromosomal aberrations and rearrangements using comparative genomic hybridization (CGH), interphase fluorescence in situ hybridization (FISH) and traditional karyotyping on several occasions while in culture. The genomic integrity was shown to be maintained after repeated freeze-thaw procedures and continuous culture in vitro for up to 22 months (148 passages). We discuss the most common de novo chromosomal aberrations reported in hESCs, as well as their possible origin.
Subject headings
- NATURVETENSKAP -- Matematik (hsv//swe)
- NATURAL SCIENCES -- Mathematics (hsv//eng)
Keyword
- *Cell Culture Techniques
- Cell Line
- Cell Proliferation
- *Chromosomal Instability
- Chromosome Aberrations
- Chromosomes
- Human
- Embryo/*cytology
- Female
- Humans
- In Situ Hybridization
- Fluorescence
- Karyotyping
- Nucleic Acid Hybridization
- Retrospective Studies
- Stem Cells/*metabolism
Publication and Content Type
- ref (subject category)
- art (subject category)
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