beta(1)-Adrenoceptor antibodies induce positive inotropic response in isolated cardiomyocytes.

beta(1)-Adrenoceptor autoantibodies are present in approximately 30% of patients suffering from dilated cardiomyopathy. The inotropic effects mediated by these antibodies remain to be studied. Monoclonal antibodies were raised against a peptide corresponding to the second extracellular loop of the human beta(1)-adrenoceptor in balb/C mouse (n=6), and were characterized by enzyme immunoassay after purification by protein A. Purified immunoglobulin G from non-immunized animals (controls) did not influence Ca(2+) transient and cell shortening of rat cardiomyocytes measured by confocal-laser-scanning-microscopy. beta(1)-adrenoceptor antibodies caused a dose-related increase in Ca(2+) transient (dilution 1:2: +35.3+/-5.1%), and in cell shortening (dilution 1:2: +40.5+/-6.3%) (P<0.01 vs. controls). The effect of the beta(1)-adrenoceptor antibodies was blocked by the antigenic peptide and by the antagonist metoprolol. In addition, beta(1)-adrenoceptor antibodies induced a dose-dependent increase of the cyclic adenosine monophosphate. The inotropic response induced by isoproterenol was attenuated by the beta(1)-adrenoceptor antibody. beta(1)-adrenoceptor antibodies as partial agonists induce a specific positive inotropic effect via the protein-kinase-A-cascade.

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Fysiologi hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Physiology hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Mikrobiologi inom det medicinska området hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Microbiology in the medical area hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Cell- och molekylärbiologi hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Cell and Molecular Biology hsv//eng
Adrenergic beta-Agonists
pharmacology
Adrenergic beta-Antagonists
pharmacology
Animals
Antibodies
Monoclonal
immunology
pharmacology
Cell Size
drug effects
Cells
Cultured
Cyclic AMP
analogs & derivatives
pharmacology
Dose-Response Relationship
Drug
Heart
drug effects
physiology
Humans
Isoproterenol
pharmacology
Metoprolol
pharmacology
Mice
Mice
Inbred BALB C
Myocardial Contraction
drug effects
Myocardium
cytology
metabolism
Propanolamines
pharmacology
Rats
Receptors
Adrenergic
beta-1
chemistry
immunology
Thionucleotides
pharmacology

Mobini, Reza,1965Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Wallenberg Laboratory(Swepub:gu)xmobre (author)
Fu, Michael,1963Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin,Wallenberglaboratoriet,Institute of Internal Medicine,Wallenberg Laboratory(Swepub:gu)xfumiw (author)
Grosse, Y (author)
Stangl, V (author)
Stangl, K (author)
Thiele, A (author)
Baumann, G (author)
Felix, S B (author)
Göteborgs universitetWallenberglaboratoriet (creator_code:org_t)

By the author/editor: Staudt, A; Mobini, Reza, 19 ...; Fu, Michael, 196 ...; Grosse, Y; Stangl, V; Stangl, K; show more...; Thiele, A; Baumann, G; Felix, S B; show less...

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MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Physiology

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Microbiology in ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Cell and Molecul ...

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