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Anti-M2 muscarinic ...
Anti-M2 muscarinic receptor antibodies inhibit beta-adrenoceptor-mediated inotropic response in rat myocardium.
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Skomedal, T (author)
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- Fu, Michael, 1963 (author)
- Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Institutionen för invärtesmedicin,Wallenberg Laboratory,Institute of Internal Medicine
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- Hjalmarson, Åke, 1937 (author)
- Gothenburg University,Göteborgs universitet,Hjärt-kärlinstitutionen,Wallenberglaboratoriet,Cardiovascular Institute,Wallenberg Laboratory
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Hoebeke, Johan (author)
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Schiander, I G (author)
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Osnes, J B (author)
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(creator_code:org_t)
- 1997
- 1997
- English.
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In: European journal of pharmacology. - 0014-2999. ; 333:2-3, s. 169-75
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Abstract
Subject headings
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- The modulation of the inotropic effect by affinity-purified antibodies against a synthetic peptide corresponding to the second extracellular loop of the human muscarinic M2 receptors was studied in adult rat ventricular myocardium. These anti-muscarinic M2 receptor antibodies shifted the dose-response relationship of the beta-adrenoceptor agonist isoproterenol to higher concentrations whereas preimmune rabbit immunoglobulin G (IgG) or antibodies against the N-terminus of the beta 1-adrenoceptor had no effect. This effect of anti-muscarinic M2 receptor antibodies was fully blocked after preincubation with the antigenic peptide. No significant change of maximal inotropic response to isoproterenol was observed in the presence of anti-muscarinic M2 receptor antibodies. The anti-muscarinic M2 receptor antibodies did apparently not hamper the access of the muscarinic receptor agonist carbachol. The muscarinic receptor antagonist atropine attenuated the effect of the anti-muscarinic M2 receptor antibodies. The present study demonstrates for the first time in intact adult ventricular myocardium a specific stimulatory muscarinic activity of antibodies raised against a part of the muscarinic M2 receptor protein.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
Keyword
- Adrenergic beta-Agonists
- pharmacology
- Amino Acid Sequence
- Animals
- Antibodies
- pharmacology
- Antigen-Antibody Reactions
- drug effects
- Atropine
- pharmacology
- Carbachol
- pharmacology
- Dose-Response Relationship
- Drug
- Heart
- drug effects
- Immunoglobulin G
- pharmacology
- Isoproterenol
- pharmacology
- Molecular Sequence Data
- Muscarinic Agonists
- pharmacology
- Muscarinic Antagonists
- pharmacology
- Myocardial Contraction
- drug effects
- Peptides
- pharmacology
- Rats
- Receptor
- Muscarinic M2
- Receptors
- Adrenergic
- beta-1
- immunology
- Receptors
- Muscarinic
- immunology
- metabolism
Publication and Content Type
- ref (subject category)
- art (subject category)
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