Search: onr:"swepub:oai:gup.ub.gu.se/60681" >
Parathyroid Ca(2+)-...
Parathyroid Ca(2+)-conducting currents are modulated by muscarinic receptor agonists and antagonists.
-
Chang, W (author)
-
Chen, T H (author)
-
Pratt, S A (author)
-
show more...
-
Yen, B (author)
-
- Fu, Michael, 1963 (author)
- Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Wallenberg Laboratory
-
Shoback, D (author)
-
show less...
-
(creator_code:org_t)
- 1997
- 1997
- English.
-
In: The American journal of physiology. - 0002-9513. ; 273:5 Pt 1
- Related links:
-
https://gup.ub.gu.se...
Abstract
Subject headings
Close
- Parathyroid cells express Ca(2+)-conducting cation currents, which are activated by raising the extracellular Ca2+ concentration ([Ca2+]o) and blocked by dihydropyridines. We found that acetylcholine (ACh) inhibited these currents in a reversible, dose-dependent manner (50% inhibitory concentration approximately equal to 10(-8) M). The inhibitory effects could be mimicked by the agonist (+)-muscarine. The effects of ACh were blunted by the antagonist atropine and reversed by removing ATP from the pipette solution (+)-Muscarine enhanced the adenosine 3',5'-cyclic monophosphate (cAMP) production by 30% but had no effect on inositol phosphate accumulation in parathyroid cells. Oligonucleotide primers, based on sequences of known muscarinic receptors (M1-M5), were used in reverse transcriptase-polymerase chain reaction (RT-PCR) to amplify receptor cDNA from parathyroid poly (A)+ RNA. RT-PCR products displayed > 90% nucleotide sequence identity to human M2- and M4-receptor cDNAs. Expression of M2-receptor protein was further confirmed by immunoblotting and immunocytochemistry. Thus parathyroid cells express muscarinic receptors of M2 and possibly M4 subtypes. These receptors may couple to dihydropyridine-sensitive, cation-selective currents through the activation of adenylate cyclase and ATP-dependent pathways in these cells.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
Keyword
- Acetylcholine
- pharmacology
- Amino Acid Sequence
- Animals
- Atropine
- pharmacology
- Base Sequence
- Calcium Channels
- biosynthesis
- drug effects
- physiology
- Cattle
- Cells
- Cultured
- Cyclic AMP
- metabolism
- DNA Primers
- Humans
- Inositol Phosphates
- metabolism
- Kinetics
- Membrane Potentials
- drug effects
- physiology
- Molecular Sequence Data
- Muscarine
- pharmacology
- Muscarinic Agonists
- pharmacology
- Muscarinic Antagonists
- pharmacology
- Nifedipine
- pharmacology
- Parathyroid Glands
- drug effects
- physiology
- Polymerase Chain Reaction
- Receptors
- Muscarinic
- biosynthesis
- genetics
- Sequence Alignment
- Sequence Homology
- Amino Acid
- Sequence Homology
- Nucleic Acid
- Tetraethylammonium
- pharmacology
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database