Beta 1 and beta 2 adrenoceptor ligand and mRNA expression in dilated cardiomyopathy.

• beta 1 and beta 2 adrenoceptor ligand activity has been shown to be down-regulated in failing myocardium. It is the aim of this study to test the hypothesis that also mRNA levels are down-regulated in dilated cardiomyopathy. beta 1 and beta 2 adrenoceptor ligand activities and mRNA expressions were analyzed in left ventricular biopsies from six organ donor hearts, in papillary muscles from seven patients operated on for mitral regurgitation, and in six explanted hearts as the result of dilated cardiomyophathy. mRNA levels were determined by solution hybridization. beta 1 ligand activity was decreased in the cases of mitral regurgitation (p < 0.01) and dilated cardiomyopathy (p < 0.001). beta 2 ligand activity did not differ between the three groups. mRNA expression was depressed in mitral regurgitation regarding both beta 1 (p < 0.001) and beta 2 (p < 0.01), while no differences were observed in dilated cardiomyopathy as compared to the donor hearts. The regulation of beta 1 and beta 2 adrenoceptor ligand activity and mRNA expression appears to follow a specific pattern in dilated cardiomyopathy. The specific down-regulation of beta 1 ligand activity seems to occur at a posttranslational level.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Physiology (hsv//eng)

Keyword

Adipose Tissue

metabolism

Adolescent

Adrenergic beta-Antagonists

pharmacology

Adult

Aged

Base Sequence

Cardiomyopathy

Dilated

metabolism

Down-Regulation

physiology

Humans

In Situ Hybridization

Ligands

Liver

metabolism

Middle Aged

Mitral Valve Prolapse

metabolism

Molecular Sequence Data

Myocardium

metabolism

Papillary Muscles

metabolism

RNA

Messenger

biosynthesis

genetics

Receptors

Adrenergic

beta-1

biosynthesis

metabolism

Receptors

Adrenergic

beta-2

biosynthesis

metabolism

Publication and Content Type

ref (subject category)

art (subject category)