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Diabetes-induced ch...
Diabetes-induced changes in the Gi-modulated muscarinic receptor-adenylyl cyclase system in rat myocardium.
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- Fu, Michael, 1963 (author)
- Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Wallenberg Laboratory
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- Bergh, Claes-Håkan, 1951 (author)
- Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Hjärt-kärlinstitutionen,Wallenberg Laboratory,Cardiovascular Institute
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- Liang, Qi-Ming (author)
- Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Wallenberg Laboratory
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- Sjögren, Klas-Göran (author)
- Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Wallenberg Laboratory
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- Xu, X (author)
- Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Wallenberg Laboratory
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Eriksson, P (author)
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Hoebeke, Johan (author)
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- Hjalmarson, Åke, 1937 (author)
- Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Wallenberg Laboratory
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(creator_code:org_t)
- 1994
- 1994
- English.
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In: Pharmacology & toxicology. - 0901-9928. ; 75:3-4, s. 186-93
- Related links:
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Abstract
Subject headings
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- The inhibitory guanine nucleotide binding regulatory protein (Gi)-mediated muscarinic receptor-adenylyl cyclase system was studied in myocardium from adult male Wistar rats with 10 weeks of diabetes induced by a single intravenous injection of streptozotocin (60 mg/kg). Neither the messenger ribonucleic acid level nor the amount of Gi was changed in the streptozotocin diabetic group as compared to the control group. The activity of the adenylyl cyclase stimulated by guanyliminodiphosphate was decreased by 48% in the streptozotocin diabetic group whereas stimulated activities of adenylyl cyclase by sodium fluoride and forskolin remained unchanged. The inhibition of forskolin-stimulated adenylyl cyclase activity by carbachol was more potent in membranes from the streptozotocin diabetic group than that in membranes from the control group. The competition binding curve between (3H)- quinuclidinyl benzilate and carbachol obtained from the streptozotocin diabetic group was shifted to the left as compared to the control group. These results suggest that the myocardium of streptozotocin-induced diabetic rats exhibited an increase in Gi function as demonstrated by the increased inhibition of guanyliminodiphosphate-mediated adenylyl cyclase and the superhigh affinity for carbachol of the muscarinic receptors. As there were signs, similar to those seen in clinical heart failure, in the streptozotocin diabetic group, these results demonstrate that functional alteration of Gi might underlie, at least in part, the cardiac dysfunction that is associated with diabetes.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
Keyword
- Adenylate Cyclase
- metabolism
- Amino Acid Sequence
- Animals
- Blotting
- Northern
- Body Weight
- Cell Membrane
- Diabetes Mellitus
- Experimental
- metabolism
- Enzyme-Linked Immunosorbent Assay
- GTP-Binding Proteins
- chemistry
- genetics
- metabolism
- Guanylyl Imidodiphosphate
- metabolism
- Male
- Molecular Sequence Data
- Myocardium
- metabolism
- Peptide Fragments
- chemistry
- RNA
- Messenger
- metabolism
- Rats
- Rats
- Wistar
- Receptors
- Muscarinic
- metabolism
Publication and Content Type
- ref (subject category)
- art (subject category)
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Fu, Michael, 196 ...
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Bergh, Claes-Håk ...
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Liang, Qi-Ming
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Sjögren, Klas-Gö ...
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Xu, X
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Eriksson, P
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Hoebeke, Johan
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Hjalmarson, Åke, ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Physiology
- Articles in the publication
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Pharmacology & t ...
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University of Gothenburg