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Hypersensitivity of...
Hypersensitivity of Gi protein mediated muscarinic receptor adenylyl cyclase in chronic ischaemic heart failure in the rat.
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- Fu, Michael, 1963 (author)
- Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Wallenberg Laboratory
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- Feng, Qing-Ping (author)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för klinisk farmakologi,Institute of Internal Medicine, Dept of Clinical Pharmacology
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- Liang, Qi-Ming (author)
- Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Wallenberg Laboratory
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- Sun, Xiang-Ying (author)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för klinisk farmakologi,Institute of Internal Medicine, Dept of Clinical Pharmacology
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- Hedner, Thomas, 1949 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för klinisk farmakologi,Institute of Internal Medicine, Dept of Clinical Pharmacology
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Hoebeke, Johan (author)
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- Hjalmarson, Åke, 1937 (author)
- Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Wallenberg Laboratory
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(creator_code:org_t)
- 1993
- 1993
- English.
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In: Cardiovascular research. - 0008-6363. ; 27:11, s. 2065-70
- Related links:
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https://gup.ub.gu.se...
Abstract
Subject headings
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- OBJECTIVE: The aim was to study the Gi protein mediated muscarinic signalling system in the myocardium of rats with chronic ischaemic heart failure. METHODS: Chronic ischaemic heart failure was induced by myocardial ischaemia (four weeks after coronary artery ligation) in rats. The densities and agonist affinities of muscarinic receptors, and the functional activity and concentration of Gi proteins were studied. RESULTS: In failing hearts, the activity of adenylyl cyclase stimulated by guanyliminodiphosphate (Gpp(NH)p) was decreased by 46%. Stimulated activities of adenylyl cyclase by both sodium fluoride and forskolin, however, remained unchanged. Carbachol depressed forskolin stimulated adenylyl cyclase more in membranes from failing hearts than those from normal hearts. The functional level of Gs protein as measured by a reconstitution assay in sarcolemmal membrane did not differ between the two groups. Furthermore, muscarinic receptors exhibited superhigh and low affinities for agonist in failing hearts whereas those in control hearts displayed only high and low affinities. No significant difference in the peptide equivalent amount of membrane bound Gi protein was found in either group. CONCLUSIONS: The experimental chronic failing heart due to myocardial ischaemia showed a depressed myocardial adenylyl cyclase signalling system. This may be due to the hypersensitivity of the Gi protein mediated muscarinic receptor-adenylyl cyclase system as shown by the increased inhibition of Gpp(NH)p mediated adenylyl cyclase, more potent inhibition of stimulated adenylyl cyclase by carbachol, and the superhigh affinity of the muscarinic receptors for carbachol.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Adenylate Cyclase
- metabolism
- Animals
- Carbachol
- pharmacology
- Cell Membrane
- drug effects
- Enzyme-Linked Immunosorbent Assay
- Forskolin
- pharmacology
- GTP-Binding Proteins
- metabolism
- Guanylyl Imidodiphosphate
- pharmacology
- Immunoblotting
- Male
- Myocardial Ischemia
- metabolism
- Myocardium
- cytology
- metabolism
- Rats
- Rats
- Sprague-Dawley
- Receptors
- Muscarinic
- metabolism
- Sarcolemma
- metabolism
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Fu, Michael, 196 ...
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Feng, Qing-Ping
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Liang, Qi-Ming
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Sun, Xiang-Ying
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Hedner, Thomas, ...
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Hoebeke, Johan
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show more...
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Hjalmarson, Åke, ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
- Articles in the publication
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Cardiovascular r ...
- By the university
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University of Gothenburg