High Osmolarity and L-DOPA Augment Release via the Fusion Pore in PC12 Cells

Sombers, L A (author)

Maxson, M M (author)

Wittenberg, N J (author)

Ewing, Andrew G, 1957 (author): Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg

(creator_code:org_t)

Wiley, 2007
2007
English.
In: Chemphyschem. - : Wiley. - 1439-4235 .- 1439-7641. ; 8:17, s. 2471-2477

Related links:: https://gup.ub.gu.se...; show more...; https://doi.org/10.1...; https://research.cha...; show less...

Abstract Subject headings

We have amperometrically measured dopamine release from rat pheochromocytoma cells (PC12 cells) in high osmolarity conditions with and without L-3,4-dihydroxyphenylalanine (L-DOPA) treatment. We observe an increase in the number of release events displaying a prespike feature or foot when the cells are stimulated in high osmolarity saline. We also see an increase in foot area and duration when cells are stimulated in high osmolarity saline, or high osmolarity saline subsequent to incubation with the dopamine precursor L-DOPA in isotonic saline, which serves to increase the vesicle size. The data suggest that membrane biophysics are an important component in defining the rate, duration and amount of neurotransmitter release via the fusion pore.

By the author/editor: Sombers, L A; Maxson, M M; Wittenberg, N J; Ewing, Andrew G, ...

About the subject

By the university: University of Gothenburg; Chalmers University of Technology

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.