Localization of Sphingomyelin in Cholesterol Domains by Imaging Mass Spectrometry

McQuaw, Carolyn M (author)

Zheng, Leiliang (author)

Ewing, Andrew G, 1957 (author): Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg

Winograd, Nicholas (author)

(creator_code:org_t)

Related links:: https://gup.ub.gu.se...; show more...; https://research.cha...; show less...

Abstract Subject headings

The location of each lipid in a palmitoyloleoylphosphatidylcholine/18:0 sphingomyelin/cholesterol monolayer system is laterally resolved using imaging time-of-flight secondary ion mass spectrometry (TOF-SIMS) without the necessity of adding fluorescent labels. This system of coexisting immiscible liquid phases shows cholesterol domains with sizes and shapes comparable to those in the fluorescence microscopy literature. The results show that SM localizes with cholesterol and that palmitoyloleoylphosphatidylcholine is excluded. Moreover, the segregation is not complete, and there is a small amount of both phospholipids distributed throughout.

By the author/editor: McQuaw, Carolyn ...; Zheng, Leiliang; Ewing, Andrew G, ...; Winograd, Nichol ...

About the subject

By the university: University of Gothenburg; Chalmers University of Technology

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.