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The role of catheps...
The role of cathepsin B and cystatin C in the mechanisms of invasion by ovarian cancer
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Nishikawa, H. (author)
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Ozaki, Y. (author)
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Nakanishi, T. (author)
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- Blomgren, Klas, 1963 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för fysiologi och farmakologi, Avdelningen för fysiologi,Institutionen för kvinnors och barns hälsa, Avdelningen för pediatrik,Institute of Physiology and Pharmacology, Dept of Physiology,Institute for the Health of Women and Children, Dept of Paediatrics
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Tada, T. (author)
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Arakawa, A. (author)
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Suzumori, K. (author)
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(creator_code:org_t)
- Elsevier BV, 2004
- 2004
- English.
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In: Gynecol Oncol. - : Elsevier BV. - 0090-8258. ; 92:3, s. 881-6
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https://doi.org/10.1...
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Abstract
Subject headings
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- OBJECTIVE: The aim of this study was to investigate the contribution of cathepsin B and cystatin C to the mechanisms of invasion by ovarian cancer. MATERIALS AND METHODS: Using surgical materials from patients with ovarian cancer, immunohistochemistry, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting analysis were performed using antibodies against cathepsin B or cystatin C. Serum levels of cathepsin B and cystatin C in patients with benign and malignant ovarian lesions were determined by enzyme-linked immunosorbent assay (ELISA). An invasion assay using an ovarian cancer cell line was performed by addition of cystatin C or specific inhibitors of cathepsin B. RESULTS: While immunohistochemical staining of cathepsin B and cystatin C was evident in cancer cells and associated stromal tissue, this was not the case in benign tumors. The malignancies were also found to be positive for cathepsin B and cystatin C by SDS-PAGE and Western blotting analysis. No significant difference in serum cathepsin B levels was observed between patients with benign and malignant disease. However, the concentration of cystatin C in cases with ovarian cancer was significantly higher in benign cases (P<0.0001) and in healthy controls (P<0.0001). Invasion by cancer cells was dose-dependently suppressed by cystatin C and cathepsin B inhibitors. CONCLUSION: The results provided convincing evidence that cathepsin B and cystatin C may contribute to the mechanisms of invasion of ovarian cancer.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Adolescent
- Adult
- Aged
- Blotting
- Western
- Cathepsin B/blood/*physiology
- Cell Adhesion/physiology
- Cell Division/physiology
- Cell Line
- Tumor
- Cystatins/blood/*physiology
- Electrophoresis
- Polyacrylamide Gel
- Enzyme-Linked Immunosorbent Assay
- Female
- Humans
- Immunohistochemistry
- Middle Aged
- Neoplasm Invasiveness
- Ovarian Neoplasms/*enzymology/*pathology
Publication and Content Type
- ref (subject category)
- art (subject category)
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