The functional significance of absence: the chromosomal segment harboring Tp53 is absent from the T55 rat radiation hybrid mapping panel.

Behboudi, Afrouz, 1967 (author): Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi,Department of Cell and Molecular Biology,Department of Cell and Molecular Biology-Genetics, Lundberg Laboratory, Göteborg University, Sweden

Roshani, Leyla, 1972 (author): Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi,Department of Cell and Molecular Biology,Department of Cell and Molecular Biology-Genetics, Lundberg Laboratory, Göteborg University, Sweden

Lundin, Lisa (author): Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi,Department of Cell and Molecular Biology,Department of Cell and Molecular Biology-Genetics, Lundberg Laboratory, Göteborg University, Sweden

Ståhl, Fredrik, 1956 (author): Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi, genetik,Department of Cell and Molecular Biology, Genetics,Department of Cell and Molecular Biology-Genetics, Lundberg Laboratory, Göteborg University, Sweden

Klinga-Levan, Karin, 1951- (author): Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi,Department of Cell and Molecular Biology,Department of Cell and Molecular Biology-Genetics, Lundberg Laboratory, Göteborg University, Sweden

Levan, Göran, 1939 (author): Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi, genetik,Department of Cell and Molecular Biology, Genetics,Department of Cell and Molecular Biology-Genetics, Lundberg Laboratory, Göteborg University, Sweden

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Abstract Subject headings

The T55 rat radiation hybrid (RH) mapping panel has been reported to retain the entire rat genome at retention frequencies between 22% and 37%. However, we found that a small segment of rat chromosome 10 harboring at least four different genes, including Tp53, was completely absent from the panel (retention frequency = 0%). Two other markers located in the vicinity exhibited much reduced retention (2-6%). RH clones are generated by transferring highly fragmented DNA into a recipient cell. There might be a strong selection against the transfer and retention of chromosome segments harboring an intact Tp53, as the action of this gene might prevent proliferation and establishment of the RH clone. Our finding further suggests that unexpected low retention or absence of chromosome segments in an RH panel may represent indications that the segments harbor genes with important functions in cell proliferation control.

By the author/editor: Behboudi, Afrouz ...; Roshani, Leyla, ...; Lundin, Lisa; Ståhl, Fredrik, ...; Klinga-Levan, Ka ...; Levan, Göran, 19 ...

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