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A Lipidic-Sponge Ph...
A Lipidic-Sponge Phase Screen for Membrane Protein Crystallization
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- Wöhri, Annemarie, 1976 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Johansson, Linda C, 1983 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
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Wadsten-Hindrichsen, Pia, 1969 (author)
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- Wahlgren, Weixiao Yuan, 1970 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
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- Fischer, Gerhard, 1978 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
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- Horsefield, Rob, 1977 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
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- Katona, Gergely, 1975 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
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- Nyblom, Anna Maria, 1975 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Öberg, Fredrik, 1982 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
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Young, G (author)
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Cogdell, Richard (author)
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Fraser, N J (author)
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- Engström, Sven, 1951 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Neutze, Richard, 1969 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
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- Hindrichsen, Pia Jeanette, 1969 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- Elsevier BV, 2008
- 2008
- English.
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In: Structure. - : Elsevier BV. - 0969-2126 .- 1878-4186. ; 16:7, s. 1003-1009
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Abstract
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- A major current deficit in structural biology is the lack of high-resolution structures of eukaryotic membrane proteins, many of which are key drug targets for the treatment of disease. Numerous eukaryotic membrane proteins require specific lipids for their stability and activity, and efforts to crystallize and solve the structures of membrane proteins that do not address the issue of lipids frequently end in failure rather than success. To help address this problem, we have developed a sparse matrix crystallization screen consisting of 48 lipidic-sponge phase conditions. Sponge phases form liquid lipid bilayer environments which are suitable for conventional hanging- and sitting-drop crystallization experiments. Using the sponge phase screen, we obtained crystals of several different membrane proteins from bacterial and eukaryotic sources. We also demonstrate how the screen may be manipulated by incorporating specific lipids such as cholesterol; this modification led to crystals being recovered from a bacterial photosynthetic core complex.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
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- ref (subject category)
- art (subject category)
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Structure
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- By the author/editor
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Wöhri, Annemarie ...
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Johansson, Linda ...
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Wadsten-Hindrich ...
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Wahlgren, Weixia ...
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Fischer, Gerhard ...
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Horsefield, Rob, ...
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show more...
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Katona, Gergely, ...
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Nyblom, Anna Mar ...
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Öberg, Fredrik, ...
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Young, G
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Cogdell, Richard
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Fraser, N J
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Engström, Sven, ...
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Neutze, Richard, ...
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Hindrichsen, Pia ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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Structure
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University of Gothenburg
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Chalmers University of Technology