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A Lipidic-Sponge Ph...
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Wöhri, Annemarie,1976Chalmers tekniska högskola,Chalmers University of Technology
(author)
A Lipidic-Sponge Phase Screen for Membrane Protein Crystallization
- Article/chapterEnglish2008
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LIBRIS-ID:oai:gup.ub.gu.se/72384
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https://gup.ub.gu.se/publication/72384URI
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https://doi.org/10.1016/j.str.2008.06.003DOI
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https://research.chalmers.se/publication/72384URI
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
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A major current deficit in structural biology is the lack of high-resolution structures of eukaryotic membrane proteins, many of which are key drug targets for the treatment of disease. Numerous eukaryotic membrane proteins require specific lipids for their stability and activity, and efforts to crystallize and solve the structures of membrane proteins that do not address the issue of lipids frequently end in failure rather than success. To help address this problem, we have developed a sparse matrix crystallization screen consisting of 48 lipidic-sponge phase conditions. Sponge phases form liquid lipid bilayer environments which are suitable for conventional hanging- and sitting-drop crystallization experiments. Using the sponge phase screen, we obtained crystals of several different membrane proteins from bacterial and eukaryotic sources. We also demonstrate how the screen may be manipulated by incorporating specific lipids such as cholesterol; this modification led to crystals being recovered from a bacterial photosynthetic core complex.
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Johansson, Linda C,1983Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg(Swepub:gu)xjlinf
(author)
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Wadsten-Hindrichsen, Pia,1969
(author)
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Wahlgren, Weixiao Yuan,1970Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg(Swepub:gu)xyuawe
(author)
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Fischer, Gerhard,1978Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg(Swepub:gu)xfisge
(author)
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Horsefield, Rob,1977Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg(Swepub:gu)xhorro
(author)
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Katona, Gergely,1975Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg(Swepub:gu)xkatge
(author)
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Nyblom, Anna Maria,1975Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)mbtkmn
(author)
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Öberg, Fredrik,1982Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg(Swepub:gu)xobefr
(author)
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Young, G
(author)
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Cogdell, Richard
(author)
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Fraser, N J
(author)
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Engström, Sven,1951Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)sv
(author)
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Neutze, Richard,1969Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg(Swepub:cth)xneuri
(author)
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Hindrichsen, Pia Jeanette,1969Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)wadsten
(author)
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Chalmers tekniska högskolaInstitutionen för kemi
(creator_code:org_t)
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In:Structure: Elsevier BV16:7, s. 1003-10090969-21261878-4186
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