A gene expression fingerprint of mouse stomach ECL cells.

• Many of the endocrine cells in the stomach are poorly characterized with respect to physiological significance. In some cases, the anticipated hormone has not yet been identified. Global gene expression analysis of mouse stomach was performed in an attempt to identify the ECL-cell peptide/protein. Specific functional activation (omeprazole-induced hypergastrinaemia) was used as a tool to generate a gene expression fingerprint of the ECL cells. The proposed fingerprint includes 14 genes, among them six are known to be expressed by ECL cells (=positive controls), and some novel ones, which are likely to be ECL-cell-related. The known ECL-cell-related genes are those encoding histidine decarboxylase, chromogranin A and B, vesicular monoamine transporter 2, synaptophysin, and the cholecystokinin-B receptor. In addition, the fingerprint included five genes, which might be involved in the process of secretion and three ESTs with unknown function. Interestingly, parathyroid hormone-like hormone (Pthlh) was identified as a candidate ECL-cell peptide hormone.

Subject headings

NATURVETENSKAP  -- Biologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences (hsv//eng)

Keyword

Animals

Cells

Cultured

Enteroendocrine Cells

metabolism

Female

Gastric Mucosa

metabolism

Gastrins

metabolism

Mice

Mice

Inbred C57BL

Omeprazole

Peptide Hormones

metabolism

Peptide Mapping

methods

Proteome

metabolism

Stomach

metabolism

Stomach Diseases

chemically induced

metabolism

Tissue Distribution

Stomach

enterochromaffin-like cells

microarray

inbred C57BL/6

mice

stomach

gastric mucosa

ECL cells

parathyroid hormone-like hormone

parathyroid hormone-related peptide

Publication and Content Type

ref (subject category)

art (subject category)