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Possible roles of i...
Possible roles of insulin-like growth factor in regulation of physiological and pathophysiological liver growth.
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- Skrtic, Stanko, 1970 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för klinisk farmakologi,Institute of Internal Medicine, Dept of Clinical Pharmacology
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- Wallenius, Kristina, 1973 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin,Institute of Internal Medicine
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- Sjögren, Klara, 1970 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för internmedicin,Institute of Internal Medicine, Dept of Medicine
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- Isaksson, Olle, 1943 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin,Institute of Internal Medicine
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- Ohlsson, Claes, 1965 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för internmedicin,Institute of Internal Medicine, Dept of Medicine
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- Jansson, John-Olov, 1954 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin,Institute of Internal Medicine
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(creator_code:org_t)
- 2001
- 2001
- English.
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In: Hormone research. - 0301-0163. ; 55 Suppl 1, s. 1-6
- Related links:
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https://gup.ub.gu.se...
Abstract
Subject headings
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- BACKGROUND/AIMS: Almost all circulating insulin-like growth factor-1 (IGF-1) is produced and secreted from the liver. However, the possible role of IGF-1 in local regulation of liver functions including liver growth is unclear. In the present study, we investigated the role of IGF-1 on liver growth in vivo and in hepatic stellate cell function in vitro. RESULTS: Liver-specific knock-out of the IGF-1 gene by use of the cre-loxP system caused enhanced liver growth, possibly reflecting increased growth hormone (GH) secretion due to decreased negative feedback by IGF-1. Studies on cultured rat hepatic stellate cells (HSC) showed that IGF-1 and hepatocyte-conditioned medium (PCcM) time- and dose-dependently increased hepatocyte growth factor (HGF) mRNA and HGF immunoreactivity. IGF-1 and PCcM also enhanced DNA synthesis in the HSC cultures. The PCcM did not contain bioactive IGF-1 and was also able to stimulate proliferation when prepared under serum- and hormone-free conditions. CONCLUSION: In vivo results show that IGF-1 is not essential for normal growth of the intact liver. The in vitro results indicate that both IGF-1 and IGF- 1-independent factor(s) from hepatocytes can stimulate HGF production by HSC. It remains to be investigated whether these effects are of importance for liver regeneration or pathological conditions.
Keyword
- Animals
- Cell Division
- physiology
- Culture Media
- Conditioned
- pharmacology
- DNA
- biosynthesis
- Hepatocyte Growth Factor
- biosynthesis
- genetics
- Hepatocytes
- cytology
- pathology
- Liver
- cytology
- growth & development
- metabolism
- pathology
- Liver Diseases
- physiopathology
- Mice
- Mice
- Knockout
- genetics
- Mitogens
- pharmacology
- Somatomedins
- deficiency
- physiology
Publication and Content Type
- ref (subject category)
- art (subject category)
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Skrtic, Stanko, ...
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Wallenius, Krist ...
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Sjögren, Klara, ...
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Isaksson, Olle, ...
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Ohlsson, Claes, ...
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Jansson, John-Ol ...
- Articles in the publication
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Hormone research
- By the university
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University of Gothenburg