onr:"swepub:oai:gup.ub.gu.se/81606"
Search: onr:"swepub:oai:gup.ub.gu.se/81606" > Abnormal reactivity...
- 1 of 1
- Previous record
- Next record
- To hitlist
Abnormal reactivity of muller cells after retinal detachment in mice deficient in GFAP and vimentin.
- Verardo, Mark R (author)
- Lewis, Geoffrey P (author)
- Takeda, Masumi (author)
- show more...
- Linberg, Kenneth A (author)
- Byun, Jiyun (author)
- Luna, Gabriel (author)
- Chen, Dong-Feng (author)
- Fisher, Steven K (author)
- show less...
- (creator_code:org_t)
- Association for Research in Vision and Ophthalmology (ARVO), 2008
- 2008
- English.
- In: Investigative ophthalmology & visual science. - : Association for Research in Vision and Ophthalmology (ARVO). - 1552-5783. ; 49:8, s. 3659-65
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- PURPOSE: To determine the roles of glial fibrillary acidic protein (GFAP) and vimentin in M?ller cell reactivity. METHODS: Retinal detachments were created in mice deficient for GFAP and vimentin (GFAP(-/-)vim(-/-)) and age-matched wild-type (wt) mice. The reactivity of the retina was studied by immunofluorescence and electron microscopy. RESULTS: M?ller cell morphology was different and glutamine synthetase immunoreactivity was reduced in the undisturbed GFAP(-/-)vim(-/-) retinas. After retinal detachment, M?ller cells formed subretinal glial scars in the wt mice. In contrast, such scars were not observed in GFAP(-/-)vim(-/-) mice. M?ller cells, which normally elongate and thicken in response to detachment, appeared compressed, thin, and "spikey" in the GFAP(-/-)vim(-/-) mice. The end foot region of M?ller cells in the GFAP(-/-)vim(-/-) mice often sheared away from the rest of the retina during detachment, corroborating earlier results showing decreased resistance of this region in GFAP(-/-)vim(-/-) retinas to mechanical stress. In regions with end foot shearing, ganglion cells showed intense neurite sprouting, as revealed by anti-neurofilament labeling, a response rarely observed in wt mice. CONCLUSIONS: M?ller cells are subtly different in the GFAP(-/-)vim(-/-) mouse retina before detachment. The end foot region of these cells may be structurally reinforced by the presence of the intermediate filament cytoskeleton, and our data suggest a critical role for these proteins in M?ller cell reaction to retinal detachment and participation in subretinal gliosis.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Keyword
- Animals
- Disease Models
- Animal
- Fluorescent Antibody Technique
- Indirect
- Glutamate-Ammonia Ligase
- metabolism
- Male
- Mice
- Mice
- Inbred C57BL
- Mice
- Knockout
- Microscopy
- Confocal
- Microscopy
- Electron
- Transmission
- Nerve Tissue Proteins
- physiology
- Neuroglia
- metabolism
- pathology
- Photoreceptor Cells
- Vertebrate
- metabolism
- pathology
- Retina
- metabolism
- pathology
- Retinal Bipolar Cells
- metabolism
- pathology
- Retinal Detachment
- metabolism
- pathology
- Retinal Ganglion Cells
- metabolism
- pathology
- Retinal Horizontal Cells
- metabolism
- pathology
- Rod Opsins
- metabolism
- S100 Proteins
- metabolism
- Up-Regulation
- Vimentin
- physiology
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Investigative ophthalmology & visual science (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Verardo, Mark R
- Lewis, Geoffrey ...
- Takeda, Masumi
- Linberg, Kenneth ...
- Byun, Jiyun
- Luna, Gabriel
- show more...
- Wilhelmsson, Ulr ...
- Pekny, Milos, 19 ...
- Chen, Dong-Feng
- Fisher, Steven K
- show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Basic Medicine
- and Neurosciences
- Articles in the publication
- Investigative op ...
- By the university
- University of Gothenburg
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
