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  • Danielsen, Pernille Hogh (author)

Oxidatively damaged DNA and its repair after experimental exposure to wood smoke in healthy humans.

  • Article/chapterEnglish2008

Publisher, publication year, extent ...

  • Elsevier BV,2008

Numbers

  • LIBRIS-ID:oai:gup.ub.gu.se/82126
  • https://gup.ub.gu.se/publication/82126URI
  • https://doi.org/10.1016/j.mrfmmm.2008.04.001DOI
  • https://research.chalmers.se/publication/82126URI

Supplementary language notes

  • Language:English

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Particulate matter from wood smoke may cause health effects through generation of oxidative stress with resulting damage to DNA. We investigated oxidatively damaged DNA and related repair capacity in peripheral blood mononuclear cells (PBMC) and measured the urinary excretion of repair products after controlled short-term exposure of human volunteers to wood smoke. Thirteen healthy adults were exposed first to clean air and then to wood smoke in a chamber during 4h sessions, 1 week apart. Blood samples were taken 3h after exposure and on the following morning, and urine was collected after exposure, from bedtime until the next morning. We measured the levels of DNA strand breaks (SB), oxidized purines as formamidopyrimidine-DNA-glycosylase (FPG) sites and activity of oxoguanine glycosylase 1 (hOGG1) in PBMC by the comet assay, whereas mRNA levels of hOGG1, nucleoside diphosphate linked moiety X-type motif 1 (hNUDT1) and heme oxygenase 1 (hHO1) were determined by real-time RT-PCR. The excretion of 8-oxo-7,8-dihydro-oxoguanine (8-oxoGua) and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in urine was measured by high performance liquid chromatography purification followed by gas chromatography with mass spectrometry. The morning following exposure to wood smoke the PBMC levels of SB were significantly decreased and the mRNA levels of hOGG1 significantly increased. FPG sites, hOGG1 activity, expression of hNUDT1 and hHO1, urinary excretion of 8-oxodG and 8-oxoGua did not change significantly. Our findings support that exposure to wood smoke causes systemic effects, although we could not demonstrate genotoxic effects, possibly explained by enhanced repair and timing of sampling.

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  • Bräuner, Elvira Vaclavik (author)
  • Barregård, Lars,1948Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för samhällsmedicin och folkhälsa,Institute of Medicine, School of Public Health and Community Medicine,University of Gothenburg(Swepub:gu)xbarrl (author)
  • Sällsten, Gerd,1952Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för samhällsmedicin och folkhälsa,Institute of Medicine, School of Public Health and Community Medicine,University of Gothenburg(Swepub:gu)xsalge (author)
  • Wallin, MariaGothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för samhällsmedicin och folkhälsa,Institute of Medicine, School of Public Health and Community Medicine,University of Gothenburg(Swepub:cth)claessom (author)
  • Olinski, Ryszard (author)
  • Rozalski, Rafal (author)
  • Moller, Peter (author)
  • Loft, Steffen (author)
  • Göteborgs universitetInstitutionen för medicin, avdelningen för samhällsmedicin och folkhälsa (creator_code:org_t)

Related titles

  • In:Mutation research: Elsevier BV642:1-2, s. 37-420027-5107

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