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Cardiomyogenic gene...
Cardiomyogenic gene expression profiling of differentiating human embryonic stem cells.
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- Synnergren, Jane, 1967 (author)
- Högskolan i Skövde,Institutionen för vård och natur
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- Adak, Sudeshna (author)
- GE John F Welch Technol Ctr Export Promot Ind Pk, Bangalore, Karnataka, India
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- Englund, Mikael C. O., 1971 (author)
- Celllartis AB, SE-43146 Gothenburg, Sweden
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- Giesler, Theresa L (author)
- GE Healthcare, Piscataway, NJ 08855 USA
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- Noaksson, Karin (author)
- Celllartis AB, SE-43146 Gothenburg, Sweden
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- Lindahl, Anders, 1954 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin,Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine,Sahlgrens Univ Hosp, Dept Clin Chem Transfus Med, SE-41345 Gothenburg, Sweden
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- Nilsson, Patric (author)
- Högskolan i Skövde,Institutionen för vård och natur
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- Nelson, Deirdre (author)
- GE Global Res Ctr, Moscow 123098, Russia
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- Abbot, Stewart (author)
- GE Global Res Ctr, Moscow 123098, Russia
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Olsson, Bj?rn (author)
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- Sartipy, Peter (author)
- Celllartis AB, SE-43146 Gothenburg, Sweden
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- Olsson, Björn (author)
- Högskolan i Skövde,Institutionen för vård och natur
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(creator_code:org_t)
- Elsevier BV, 2008
- 2008
- English.
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In: Journal of biotechnology. - : Elsevier BV. - 0168-1656 .- 1873-4863. ; 134:1-2, s. 162-70
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https://doi.org/10.1...
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Abstract
Subject headings
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- Human embryonic stem cells (hESCs) can differentiate into a variety of specialized cell types. Thus, they provide a model system for embryonic development to investigate the molecular processes of cell differentiation and lineage commitment. The development of the cardiac lineage is easily detected in mixed cultures by the appearance of spontaneously contracting areas of cells. We performed gene expression profiling of undifferentiated and differentiating hESCs and monitored 468 genes expressed during cardiac development and/or in cardiac tissue. Their transcription during early differentiation of hESCs through embryoid bodies (EBs) was investigated and compared with spontaneously differentiating hESCs maintained on feeders in culture without passaging (high-density (HD) protocol). We observed a larger variation in the gene expression between cells from a single cell line that were differentiated using two different protocols than in cells from different cell lines that were cultured according to the same protocol. Notably, the EB protocol resulted in more reproducible transcription profiles than the HD protocol. The results presented here provide new information about gene regulation during early differentiation of hESCs with emphasis on the cardiomyogenic program. In addition, we also identified regulatory elements that could prove critical for the development of the cardiomyocyte lineage.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
Keyword
- Cell Differentiation
- genetics
- Cell Lineage
- genetics
- Cluster Analysis
- Embryonic Stem Cells
- cytology
- metabolism
- Gene Expression Profiling
- methods
- Humans
- Myocytes
- Cardiac
- cytology
- metabolism
- Differentiation
- Bioinformatics
- Naturvetenskap
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Synnergren, Jane ...
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Adak, Sudeshna
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Englund, Mikael ...
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Giesler, Theresa ...
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Noaksson, Karin
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Lindahl, Anders, ...
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show more...
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Nilsson, Patric
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Nelson, Deirdre
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Abbot, Stewart
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Olsson, Bj?rn
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Sartipy, Peter
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Olsson, Björn
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Bioinformatics a ...
- Articles in the publication
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Journal of biote ...
- By the university
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University of Gothenburg
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University of Skövde