onr:"swepub:oai:gup.ub.gu.se/82448"
Search: onr:"swepub:oai:gup.ub.gu.se/82448" > Retrograde endocann...
- 1 of 1
- Previous record
- Next record
- To hitlist
Retrograde endocannabinoid signaling at striatal synapses requires a regulated postsynaptic release step.
-
- Adermark, Louise, 1974 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry
- Lovinger, David M (author)
- (creator_code:org_t)
- 2007-12-18
- 2007
- English.
- In: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 1091-6490. ; 104:51, s. 20564-9
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Endocannabinoids (eCBs) mediate short- and long-term depression of synaptic strength by retrograde transsynaptic signaling. Previous studies have suggested that an eCB mobilization or release step in the postsynaptic neuron is involved in this retrograde signaling. However, it is not known whether this release process occurs automatically upon eCB synthesis or whether it is regulated by other synaptic factors. To address this issue, we loaded postsynaptic striatal medium spiny neurons (MSNs) with the eCBs anandamide (AEA) or 2-arachidonoylglycerol and determined the conditions necessary for presynaptic inhibition. We found that presynaptic depression of glutamatergic excitatory postsynaptic currents (EPSCs) and GABAergic inhibitory postsynaptic currents (IPSCs) induced by postsynaptic eCB loading required a certain level of afferent activation that varied between the different synaptic types. Synaptic depression at excitatory synapses was temperature-dependent and blocked by the eCB membrane transport blockers, VDM11 and UCM707, but did not require activation of metabotropic glutamate receptors, l-calcium channels, nitric oxide, voltage-activated Na(+) channels, or intracellular calcium. Application of the CB(1)R antagonist, AM251, after depression was established, reversed the decrease in EPSC, but not in IPSC, amplitude. Direct activation of the CB(1) receptor by WIN 55,212-2 initiated synaptic depression that was independent of afferent stimulation. These findings indicate that retrograde eCB signaling requires a postsynaptic release step involving a transporter or carrier that is activated by afferent stimulation/synaptic activation.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
Keyword
- Animals
- Arachidonic Acids
- antagonists & inhibitors
- pharmacology
- Benzoxazines
- pharmacology
- Calcium Channel Blockers
- pharmacology
- Corpus Striatum
- drug effects
- physiology
- Endocannabinoids
- antagonists & inhibitors
- pharmacology
- physiology
- Furans
- pharmacology
- Glutamic Acid
- physiology
- Glycerides
- antagonists & inhibitors
- pharmacology
- Long-Term Synaptic Depression
- drug effects
- Membrane Potentials
- Morpholines
- pharmacology
- Naphthalenes
- pharmacology
- Piperidines
- pharmacology
- Polyunsaturated Alkamides
- antagonists & inhibitors
- pharmacology
- Pyrazoles
- pharmacology
- Rats
- Receptor
- Cannabinoid
- CB1
- agonists
- antagonists & inhibitors
- Synapses
- drug effects
- physiology
- Synaptic Transmission
- gamma-Aminobutyric Acid
- physiology
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Proceedings of the National Academy of Sciences of the United States of America (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Adermark, Louise ...
- Lovinger, David ...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Basic Medicine
- and Physiology
- Articles in the publication
- Proceedings of t ...
- Proceedings of t ...
- By the university
- University of Gothenburg
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
