Characterization of neuroprogenitor cells expressing the PDGF beta-receptor within the subventricular zone of postnatal mice.

• We report a considerable number of cells in the ventricular and the subventricular zones (SVZ) of newborn mice to stain positive for the PDGF beta-receptor (PDGFRB). Many of them also stained for nestin and/or GFAP but less frequently for the neuroblast marker doublecortin and for the mitotic marker Ki-67. The SVZ of mice with nestin-Cre conditional deletion of PDGFRB expressed the receptor only on blood vessels and was devoid of any morphological abnormality. PDGFRB(-/-) neurospheres showed a higher rate of apoptosis without any significant decrease in proliferation. They demonstrated reduced capacities of migration and neuronal differentiation in response to not only PDGF-BB but also bFGF. Furthermore, the PDGFR kinase inhibitor STI571 blocked the effects of bFGF in control neurosphere cultures. bFGF increased the activity of the PDGFRB promoter as well as the expression and phosphorylation of PDGFRB. These results suggest the presence of the signaling convergence between PDGF and FGF. PDGFRB is needed for survival, and the effects of bFGF in migration and neural differentiation of the cells may be potentiated by induction of PDGFRB.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)

Keyword

Analysis of Variance

Animals

Animals

Newborn

Bromodeoxyuridine

metabolism

Cell Differentiation

drug effects

genetics

Cell Movement

drug effects

physiology

Cells

Cultured

Enzyme Inhibitors

pharmacology

Gene Expression

drug effects

physiology

Intermediate Filament Proteins

genetics

metabolism

Ki-67 Antigen

metabolism

Lateral Ventricles

cytology

growth & development

Mice

Mice

Inbred C57BL

Mice

Transgenic

Nerve Tissue Proteins

genetics

metabolism

Neurons

physiology

Piperazines

pharmacology

Pyrimidines

pharmacology

Receptor

Platelet-Derived Growth Factor beta

genetics

metabolism

Stem Cells

metabolism

Transfection

methods

Publication and Content Type

ref (subject category)

art (subject category)