Mechanism of MASH1 induction by ASK1 and ATRA in adult neural progenitors.

• The molecular mechanisms underlying differentiation and lineage commitment in neural stem cells are just beginning to be understood, however the molecules involved in this process and their functions remain largely unknown. Here we studied the effects and downstream signals of apoptosis signal-regulating kinase 1 (ASK1) together with all-trans retinoic acid (ATRA) on neuronal differentiation in adult hippocampus-derived progenitor (AHP) cells. Following ASK1 over-expression and ATRA treatment in AHPs, a larger number of cells differentiated into neurons and the MASH1 promoter became activated. Analyzing downstream effector molecules of ASK1 or ATRA targeting the MASH1 promoter revealed that the myocyte enhancer factor 2C (MEF2C) mediated ASK1 signalling, while activation of Sp1 was involved in ATRA signalling. Chromatin immunoprecipitation assay on the promoter revealed that ASK1 induced binding of MEF2C and Ca(2+)/calmodulin-dependent kinase II to the MASH1 promoter. Taken together, ASK1 and ATRA activate MEF2C and Sp1, respectively, and up-regulate MASH1 protein expression.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)

Keyword

Adult Stem Cells

drug effects

Analysis of Variance

Animals

Antineoplastic Agents

pharmacology

Basic Helix-Loop-Helix Transcription Factors

metabolism

Cell Differentiation

drug effects

Cell Line

Chromatin Immunoprecipitation

methods

Dose-Response Relationship

Drug

Enzyme Activation

drug effects

genetics

Gene Expression Regulation

Enzymologic

drug effects

Hippocampus

cytology

Immunoprecipitation

MAP Kinase Kinase Kinase 5

metabolism

Mutation

physiology

Myogenic Regulatory Factors

metabolism

Neurons

drug effects

physiology

Rats

Time Factors

Transfection

methods

Tretinoin

pharmacology

Publication and Content Type

ref (subject category)

art (subject category)