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The PDGF B-chain is...
The PDGF B-chain is involved in the ontogenic susceptibility of the developing rat brain to NMDA toxicity.
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Egawa-Tsuzuki, Tomoko (author)
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Ohno, Masaki (author)
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Tanaka, Naoto (author)
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Takeuchi, Yoshihiro (author)
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- Uramoto, Hidetaka (author)
- Gothenburg University,Göteborgs universitet,Institutionen för anatomi och cellbiologi,Institute of Anatomy and Cell Biology
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- Faigle, Roland, 1977 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för anatomi och cellbiologi,Institute of Anatomy and Cell Biology
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- Funa, Keiko, 1949 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för anatomi och cellbiologi,Institute of Anatomy and Cell Biology
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Ishii, Yoko (author)
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Sasahara, Masakiyo (author)
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(creator_code:org_t)
- Elsevier BV, 2004
- 2004
- English.
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In: Experimental neurology. - : Elsevier BV. - 0014-4886. ; 186:1, s. 89-98
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Abstract
Subject headings
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- Hypoxic-ischemic (H-I) injury to neonatal brains can cause a life-long neuronal deficit because of increased susceptibility in the neonatal period. Excitotoxicity due to overstimulation of the N-methyl-d-aspartate receptor (NMDAR) is assumed to be the basis of the injury. However, the ontogenic profile of the susceptibility does not directly correlate with the levels of NMDAR expression. Platelet-derived growth factor B-chain (PDGF-B) has been reported to protect neurons by suppressing the NMDA-evoked current and translocating the glutamate transporter to the cell membrane. Thus, we assessed the relationship between the susceptibility to H-I injury and the expression of PDGF-B in neonatal rat brain. PDGF-B infusion before and after an intrastriatal NMDA injection significantly reduced the size of the lesions in 7-day-old rats, when they are most susceptible and the neuronal expression of PDGF-B is low. Fourteen-day-old neonatal rats were found to be resistant to NMDA injury, even though NMDARs are expressed at high levels in the brain at this age. Inhibition of PDGF-B protein synthesis by antisense oligodeoxynucleotides increased the size of the NMDA-induced lesions up to 6-fold at postnatal day 14, when PDGF-B is expressed at high levels in neurons. These data suggest that PDGF-B is an important physiological modulator of NMDAR excitability in the developing brain, and that the balance between the expression of NMDAR and PDGF-B partly determines the ontogenic susceptibility to brain injury. Enhancement of the PDGF-B/receptor signal pathway might rescue neonatal brains at risk of H-I injury.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Aging
- Analysis of Variance
- Animals
- Animals
- Newborn
- Brain
- drug effects
- growth & development
- pathology
- COS Cells
- Cercopithecus aethiops
- DNA
- metabolism
- Disease Susceptibility
- Drug Interactions
- Excitatory Amino Acid Agonists
- toxicity
- Immunoblotting
- methods
- Immunohistochemistry
- methods
- N-Methylaspartate
- toxicity
- Oligoribonucleotides
- Antisense
- pharmacology
- Proto-Oncogene Proteins c-sis
- metabolism
- Rats
- Rats
- Sprague-Dawley
- Reverse Transcriptase Polymerase Chain Reaction
- methods
- Transfection
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Egawa-Tsuzuki, T ...
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Ohno, Masaki
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Tanaka, Naoto
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Takeuchi, Yoshih ...
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Uramoto, Hidetak ...
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Faigle, Roland, ...
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show more...
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Funa, Keiko, 194 ...
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Ishii, Yoko
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Sasahara, Masaki ...
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
- Articles in the publication
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Experimental neu ...
- By the university
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University of Gothenburg