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The yeast tumor sup...
The yeast tumor suppressor homologue Sro7p is required for targeting of the sodium pumping ATPase to the cell surface.
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- Wadskog, Ingrid (författare)
- Jönköping University,Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi,Department of Cell and Molecular Biology,JTH, Kemiteknik
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- Forsmark, Annabelle, 1973 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi,Department of Cell and Molecular Biology
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Rossi, Guendalina (författare)
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Konopka, Catherine (författare)
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Oyen, Mattias (författare)
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- Goksör, Mattias, 1975 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU)
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- Ronne, Hans (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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Brennwald, Patrick (författare)
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- Adler, Lennart, 1944 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi,Department of Cell and Molecular Biology
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(creator_code:org_t)
- 2006
- 2006
- Engelska.
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Ingår i: Molecular biology of the cell. - 1059-1524 .- 1939-4586. ; 17:12, s. 4988-5003
- Relaterad länk:
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http://www.ncbi.nlm....
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- The SRO7/SOP1 encoded tumor suppressor homologue of Saccharomyces cerevisiae is required for maintenance of ion homeostasis in cells exposed to NaCl stress. Here we show that the NaCl sensitivity of the sro7Delta mutant is due to defective sorting of Ena1p, the main sodium pump in yeast. On exposure of sro7Delta mutants to NaCl stress, Ena1p fails to be targeted to the cell surface, but is instead routed to the vacuole for degradation via the multivesicular endosome pathway. SRO7-deficient mutants accumulate post-Golgi vesicles at high salinity, in agreement with a previously described role for Sro7p in late exocytosis. However, Ena1p is not sorted into these post-Golgi vesicles, in contrast to what is observed for the vesicles that accumulate when exocytosis is blocked in sec6-4 mutants at high salinity. These observations imply that Sro7p has a previously unrecognized role for sorting of specific proteins into the exocytic pathway. Screening for multicopy suppressors identified RSN1, encoding a transmembrane protein of unknown function. Overexpression of RSN1 restores NaCl tolerance of sro7Delta mutants by retargeting Ena1p to the plasma membrane. We propose a model in which blocked exocytic sorting in sro7Delta mutants, gives rise to quality control-mediated routing of Ena1p to the vacuole.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Nyckelord
- Adenosine Triphosphatases
- metabolism
- Carrier Proteins
- metabolism
- Cation Transport Proteins
- metabolism
- Cell Membrane
- drug effects
- metabolism
- Gene Expression
- drug effects
- Genes
- Fungal
- Golgi Apparatus
- drug effects
- Mutation
- genetics
- Protein Transport
- drug effects
- Recombinant Fusion Proteins
- metabolism
- Saccharomyces cerevisiae
- cytology
- drug effects
- growth & development
- ultrastructure
- Saccharomyces cerevisiae Proteins
- metabolism
- Secretory Vesicles
- drug effects
- Sequence Homology
- Sodium Chloride
- pharmacology
- Thermodynamics
- Tumor Suppressor Proteins
- metabolism
- Vacuoles
- metabolism
- Adenosine Triphosphatases/*metabolism
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Wadskog, Ingrid
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Forsmark, Annabe ...
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Rossi, Guendalin ...
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Konopka, Catheri ...
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Oyen, Mattias
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Goksör, Mattias, ...
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visa fler...
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Ronne, Hans
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Brennwald, Patri ...
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Adler, Lennart, ...
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- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
- Artiklar i publikationen
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Molecular biolog ...
- Av lärosätet
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Göteborgs universitet
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Uppsala universitet
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Jönköping University