Search: onr:"swepub:oai:gup.ub.gu.se/87063" >
Calcium-dependent p...
Calcium-dependent proteolysis in rabbit lens epithelium after oxidative stress
-
- Andersson, Madeleine (author)
- Gothenburg University,Göteborgs universitet,Institutionen för klinisk neurovetenskap, Sektionen för oftalmologi,Institute of Clinical Neurosciences, Section of Ophtalmology
-
- Sjöstrand, Johan, 1936 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för klinisk neurovetenskap, Sektionen för oftalmologi,Institute of Clinical Neurosciences, Section of Ophtalmology
-
- Petersen, Anne, 1962 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för anatomi och cellbiologi,Institute of Anatomy and Cell Biology
-
show more...
-
- Karlsson, Jan-Olof, 1944 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för anatomi och cellbiologi,Institute of Anatomy and Cell Biology
-
show less...
-
(creator_code:org_t)
- 1998
- 1998
- English.
-
In: Ophthalmic Res. ; 30:3, s. 157-67
- Related links:
-
https://gup.ub.gu.se...
Abstract
Subject headings
Close
- The purpose of this study was to examine changes in calcium-dependent proteolytic activity in the lens epithelium from whole rabbit lenses exposed to long-term oxidative stress at near physiological levels. Rabbit lenses, incubated in 50 microM H2O2 for 1 or 24 h, were checked for clarity and morphological changes in the epithelium. Proteolytic activity was measured in the epithelium using a fluorogenic synthetic substrate; N-succinyl-Leu-Tyr-7-amino-4-methylocoumarin, both in the presence and the absence of calcium (1 mM Ca2+ and 5 mM EDTA respectively). The effect on transparency and morphology of the epithelium following a 1-hour incubation in 100 microM H2O2 was also studied. Lenses incubated in 50 microM H2O2 were clear even after 24h. After a 1-hour incubation in 50 microM H2O2 the epithelium of the exposed lens appeared normal. However, after 24 h the epithelium cells appeared swollen and microscopical examination showed extensive intracellular and subepithelial vacuolization. Incubation in 100 microM H2O2 for 1 h caused loss of transparency; vacuole formation, globulization of the superficial lens fibers and death of the epithelial cells. There was a 55% increase in calcium-dependent proteolytic activity after 1 h in 50 microM H2O2, implying a role for the calcium-activated protease calpain in oxidatively induced cataract.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Animal
- Calcium/*pharmacology
- Calpain/*metabolism
- Cataract/chemically induced/enzymology/pathology
- Epithelium/drug effects/enzymology/pathology
- Female
- Hydrogen Peroxide/toxicity
- Lens
- Crystalline/*drug effects/enzymology/pathology
- Organ Culture
- *Oxidative Stress
- Rabbits
- Support
- Non-U.S. Gov't
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database