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Identification of a...
Identification of a core set of 58 gene transcripts with broad and specific expression in the microvasculature.
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- Wallgard, Elisabet (author)
- Karolinska Institutet, Institutionen för medicinsk biokemi och biofysik
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- Larsson, Erik, 1975 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Wallenberglaboratoriet,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology,Wallenberg Laboratory
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He, Liqun (author)
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- Hellström, Mats (author)
- Karolinska Institutet, Institutionen för medicinsk biokemi och biofysik
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- Armulik, Annika (author)
- Karolinska Institutet
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Nisancioglu, Maya H (author)
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- Genove, Guillem (author)
- Karolinska Institutet
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- Lindahl, Per, 1967 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Wallenberglaboratoriet,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology,Institute of Medicine, Department of Molecular and Clinical Medicine,Wallenberg Laboratory
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- Betsholtz, Christer, 1959 (author)
- Karolinska Institutet
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(creator_code:org_t)
- 2008
- 2008
- English.
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In: Arteriosclerosis, thrombosis, and vascular biology. - 1524-4636 .- 1079-5642. ; 28:8, s. 1469-76
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Abstract
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- OBJECTIVE: Pathological angiogenesis is an integral component of many diseases. Antiangiogenesis and vascular targeting are therefore promising new therapeutic principles. However, few endothelial-specific putative drug targets have been identified, and information is still limited about endothelial-specific molecular processes. Here we aimed at determining the endothelial cell-specific core transcriptome in vivo. METHODS AND RESULTS: Analysis of publicly available microarray data identified a mixed vascular/lung cluster of 132 genes that correlated with known endothelial markers. Filtering against kidney glomerular/nonglomerular and brain vascular/nonvascular microarray profiles separated contaminating lung markers, leaving 58 genes with broad and specific microvascular expression. More than half of these have not previously been linked to endothelial functions or studied in detail before. The endothelial cell-specific expression of a selected subset of these, Eltd1, Gpr116, Ramp2, Slc9a3r2, Slc43a3, Rasip1, and NM_023516, was confirmed by real-time quantitative polymerase chain reaction and/or immunohistochemistry. CONCLUSIONS: We have used a combination of publicly available and own microarray data to identify 58 gene transcripts with broad yet specific expression in microvascular endothelium. Most of these have unknown functions, but many of them are predicted to be cell surface expressed or implicated in cell signaling processes and should therefore be explored as putative microvascular drug targets.
Subject headings
- NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Annan biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Other Biological Topics (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kardiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cardiac and Cardiovascular Systems (hsv//eng)
Keyword
- Animals
- Endothelial Cells
- Vascular
- Gene Expression Profiling
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Wallgard, Elisab ...
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Larsson, Erik, 1 ...
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He, Liqun
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Hellström, Mats
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Armulik, Annika
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Nisancioglu, May ...
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show more...
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Genove, Guillem
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Lindahl, Per, 19 ...
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Betsholtz, Chris ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Bioinformatics a ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Other Biological ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Cardiac and Card ...
- Articles in the publication
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Arteriosclerosis ...
- By the university
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University of Gothenburg
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Uppsala University
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Karolinska Institutet