Osteoprotegerin mRNA is increased by interleukin-1 alpha in the human osteosarcoma cell line MG-63 and in human osteoblast-like cells.

• Osteoprotegerin (OPG) is a soluble receptor for the Osteoprotegerin-Ligand (OPGL) which is expressed on osteoblasts and mediates the signal for osteoclast differentiation. In the present study we demonstrate that OPG mRNA levels in MG-63 cells are increased in a dose-dependent manner after 8 h of treatment with IL-1 alpha (338 +/- 53% over control at 25 U/ml). Interleukin-6 (IL-6), under similar culture conditions, does not affect OPG mRNA levels. Time-course studies show that IL-1 alpha (25 U/ml) causes a transient increase of OPG mRNA levels in MG-63 cells, peaking after 4 h of treatment. An increase of the OPG transcript occurs in hOB cells at 0.5 h which is still present after 24 h of IL-1 alpha treatment. In MG-63 cells neither basal-nor IL-1 alpha-induced OPG mRNA levels are altered by the translational inhibitor cycloheximide. These results suggest that expression of OPG in osteoblasts may be regulated by IL-1 alpha.

Nyckelord

Bone Neoplasms

Cell Differentiation

Cycloheximide

pharmacology

Dose-Response Relationship

Drug

Gene Expression Regulation

drug effects

Gene Expression Regulation

Neoplastic

drug effects

Glycoproteins

biosynthesis

Humans

Interleukin-1

pharmacology

Kinetics

Osteoblasts

drug effects

metabolism

Osteoclasts

cytology

Osteoprotegerin

Osteosarcoma

Polymerase Chain Reaction

RNA

Messenger

biosynthesis

Receptors

Cytoplasmic and Nuclear

Receptors

Tumor Necrosis Factor

biosynthesis

Signal Transduction

Transcription

Genetic

drug effects

Tumor Cells

Cultured

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