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Effects of tri-iodo...
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Ohlsson, Claes,1965Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin,Institute of Internal Medicine
(author)
Effects of tri-iodothyronine and insulin-like growth factor-I (IGF-I) on alkaline phosphatase activity, [3H]thymidine incorporation and IGF-I receptor mRNA in cultured rat epiphyseal chondrocytes.
- Article/chapterEnglish1992
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LIBRIS-ID:oai:gup.ub.gu.se/91652
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https://gup.ub.gu.se/publication/91652URI
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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The effects of tri-iodothyronine (T3) and insulin-like growth factor-I (IGF-I) on [3H]thymidine incorporation, alkaline phosphatase (ALP) activity and IGF-I receptor mRNA levels were studied in rat epiphyseal chondrocytes cultured in monolayer. Chondrocytes from enzymatically digested rat tibia epiphyseal growth plates were seeded in monolayer culture and precultured for 7-14 days in Ham's F-12 medium supplemented with 10% (v/v) newborn calf serum and 1% (v/v) of a serum substitute. After preculture the medium was changed to Ham's F-12 medium containing 1% (v/v) serum from hypophysectomized rats, and the effects of T3 and/or IGF-I on DNA synthesis ([3H]thymidine incorporation), ALP activity (a late marker of differentiated epiphyseal chondrocytes) and IGF-I receptor mRNA levels were studied. ALP activity was increased by T3 in a dose-dependent manner with a maximal response at 10 micrograms T3/l (678 +/- 86% compared with control culture). The increase in ALP activity was accompanied by a concomitant decrease in [3H]thymidine incorporation (52 +/- 14% compared with control culture). Human GH (hGH; 50 micrograms/l) and IGF-I (25 micrograms/l) had no stimulatory effect on ALP activity. However IGF-I (10 micrograms/l) exerted an inhibition on the T3 (10 micrograms/l)-induced increase in ALP activity (64 +/- 9% compared with T3-treated culture). T3 (3 micrograms/l) inhibited the increase in [3H]thymidine incorporation caused by 25 micrograms IGF-I/l (51 +/- 13% compared with IGF-I-treated culture). Furthermore, IGF-I receptor mRNA levels were increased by 10 micrograms T3/l (137 +/- 4.2% compared with control culture) while no effect of hGH (50 micrograms/l) or IGF-I (25 micrograms/l) was demonstrated. Both T3 and IGF-I were shown to interact with epiphyseal chondrocytes and both substances seemed to affect cell proliferation and maturation and therefore longitudinal bone growth. Furthermore, the results indicated that IGF-I is important for proliferation of the cells while T3 initiates the terminal differentiation of epiphyseal chondrocytes.
Subject headings and genre
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Alkaline Phosphatase
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metabolism
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Animals
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Cell Differentiation
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drug effects
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Cell Division
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drug effects
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Cells
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Cultured
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Growth Plate
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cytology
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drug effects
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metabolism
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Insulin-Like Growth Factor I
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pharmacology
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Male
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RNA
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Messenger
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analysis
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Rats
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Rats
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Sprague-Dawley
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Receptor
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IGF Type 1
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genetics
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Thymidine
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metabolism
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Triiodothyronine
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pharmacology
Added entries (persons, corporate bodies, meetings, titles ...)
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Nilsson, Anders,1958Gothenburg University,Göteborgs universitet,Institutionen för de kirurgiska disciplinerna, Avdelningen för ortopedi,Institute of Surgical Sciences, Department of Orthopaedics(Swepub:gu)xniand
(author)
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Isaksson, Olle,1943Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin,Institute of Internal Medicine(Swepub:gu)xisaol
(author)
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Bentham, J
(author)
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Lindahl, Anders,1954Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin, Avdelningen för klinisk kemi/transfusionsmedicin,Institute of Laboratory Medicine, Dept of Clinical Chemistry/Transfusion Medicine(Swepub:gu)xlandy
(author)
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Göteborgs universitetInstitutionen för invärtesmedicin
(creator_code:org_t)
Related titles
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In:The Journal of endocrinology135:1, s. 115-230022-0795
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