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Quantification and ...
Quantification and localization of the IGF/insulin system expression in retinal blood vessels and neurons during oxygen-induced retinopathy in mice
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- Löfqvist, Chatarina, 1964 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för klinisk neurovetenskap och rehabilitering,Institute of Neuroscience and Physiology, Department of Clinical Neuroscience and Rehabilitation
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Willett, K. L. (author)
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- Aspegren, Oskar (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för klinisk neurovetenskap och rehabilitering,Institute of Neuroscience and Physiology, Department of Clinical Neuroscience and Rehabilitation
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Smith, A. C. (author)
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Aderman, C. M. (author)
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Connor, K. M. (author)
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Chen, J. (author)
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- Hellström, Ann, 1959 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för klinisk neurovetenskap och rehabilitering,Institute of Neuroscience and Physiology, Department of Clinical Neuroscience and Rehabilitation
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Smith, L. E. (author)
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(creator_code:org_t)
- 2009
- 2009
- English.
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In: Invest Ophthalmol Vis Sci. - 1552-5783. ; 50:4, s. 1831-1837
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https://gup.ub.gu.se...
Abstract
Subject headings
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- PURPOSE: Retinopathy is a result of pathologic angiogenesis influenced by insulinlike growth factor (IGF)-1. The authors examined the local expression of the IGF/insulin family. METHODS: In retinas with and without oxygen-induced retinopathy, the authors assessed with real-time RT-PCR mRNA expression of the IGF-1 receptor (IGF-1R), insulin receptor (IR), IGF-1, IGF-2, insulin (Ins2), and IGF-binding protein 1 (IGFBP1) to IGFBP6 in total retina from postnatal day (P) 7 to P33 to examine changes over time with the induction of retinopathy and at P17 on laser-captured retinal components to quantitatively localize mRNA expression in the ganglion cell layer, the outer nuclear layer, the inner nuclear layer, normal blood vessels, and neovascular tufts. RESULTS: IGF-1R and IR are expressed predominantly in photoreceptors and in vessels, with scant expression in the rest of the neural retina. IGF-1R expression is more than 100-fold greater than IR. The major local growth factor (expressed in photoreceptors and in blood vessels) is IGF-2 (approximately 1000-fold greater than IGF-1). IGF-1 (approximately 600 copies/10(6) cyclophilin) is expressed throughout the retina. IGFBP2, IGFBP4, and IGFBP5 expression is unchanged with increasing retinal development and with the induction of retinopathy. In contrast, IGFBP3 expression increased more than 5-fold with hypoxia, found in neovascular tufts. CONCLUSIONS: IGF-1R, IR, and the ligand IGF-2 are expressed almost exclusively in photoreceptors and blood vessels. IGFBP3 and IGFBP5 expression increases in neovascular tufts compared with normal vessels. IGF-1 is expressed throughout the retina at much lower levels. These results suggest cross-talk between vessels and photoreceptors in the development of retinopathy and retinal vasculature.
Keyword
- Animals
- DNA Primers/chemistry
- Disease Models
- Animal
- Gene Expression Regulation
- Humans
- Infant
- Newborn
- Insulin-Like Growth Factor Binding Proteins/*genetics
- Insulin-Like Growth Factor I/*genetics
- Insulin-Like Growth Factor II/genetics
- Mice
- Mice
- Inbred C57BL
- Oxygen/*toxicity
- Photoreceptor Cells
- Vertebrate/metabolism
- RNA
- Messenger/metabolism
- Receptor
- IGF Type 1/*genetics
- Receptor
- IGF Type 2/genetics
- Receptor
- Insulin/*genetics
- Retinal Neurons/*metabolism
- Retinal Vessels/*metabolism
- Retinopathy of Prematurity/chemically induced/metabolism
- Reverse Transcriptase Polymerase Chain Reaction
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Löfqvist, Chatar ...
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Willett, K. L.
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Aspegren, Oskar
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Smith, A. C.
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Aderman, C. M.
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Connor, K. M.
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show more...
-
Chen, J.
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Hellström, Ann, ...
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Smith, L. E.
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show less...
- Articles in the publication
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Invest Ophthalmo ...
- By the university
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University of Gothenburg