Cystatin C in cerebrospinal fluid and multiple sclerosis.

• OBJECTIVE: A recent study using surface-enhanced laser desorption/ionization time-of-flight analysis of cerebrospinal fluid identified a 12.5 kDa truncated isoform of cystatin C (CysC) as a specific biomarker for multiple sclerosis (MS). METHODS: Surface-enhanced laser desorption/ionization time-of-flight analysis of cerebrospinal fluid samples from 43 MS patients and 46 healthy control subjects. RESULTS: Full-length CysC (13.4 kDa) concentration was similar in MS and control samples. The 12.5 kDa CysC protein was produced from full-length CysC by N-terminal cleavage during storage at -20 degrees C. INTERPRETATION: The 12.5 kDa CysC isoform is a storage-related artifact and is not useful as a diagnostic marker for MS.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Psykiatri (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Psychiatry (hsv//eng)

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Neurosciences (hsv//eng)

Keyword

Amino Acid Sequence

Artifacts

Biological Markers

cerebrospinal fluid

Cystatin C

Cystatins

cerebrospinal fluid

chemistry

genetics

Drug Storage

Freezing

Humans

Molecular Weight

Multiple Sclerosis

cerebrospinal fluid

Osmolar Concentration

Peptide Fragments

cerebrospinal fluid

chemistry

genetics

Protein Isoforms

cerebrospinal fluid

chemistry

genetics

Spectrometry

Mass

Matrix-Assisted Laser Desorption-Ionization

Time Factors

Publication and Content Type

ref (subject category)

art (subject category)