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In vivo electrochem...
In vivo electrochemical studies of optogenetic control of glutamate signaling measured using enzyme-based ceramic microelectrode arrays
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- Burmeister, Jason J. (author)
- University of Kentucky Medical Center
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- Pomerleau, Francois (author)
- University of Kentucky Medical Center
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- Quintero, Jorge E. (author)
- University of Kentucky Medical Center
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- Huettl, Peter (author)
- University of Kentucky Medical Center
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- Ai, Yi (author)
- University of Kentucky Medical Center
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- Jakobsson, Johan (author)
- Lund University,Lunds universitet,Molekylär neurogenetik,Forskargrupper vid Lunds universitet,Molecular Neurogenetics,Lund University Research Groups
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- Lundblad, Martin (author)
- Lund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups
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- Heuer, Andreas (author)
- Lund University,Lunds universitet,Institutionen för experimentell medicinsk vetenskap,Medicinska fakulteten,Department of Experimental Medical Science,Faculty of Medicine
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- Slevin, John T (author)
- University of Kentucky Medical Center
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- Gerhardt, Greg A. (author)
- University of Kentucky Medical Center
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(creator_code:org_t)
- 2017-09-22
- 2018
- English 25 s.
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In: Neuromethods. - New York, NY : Springer New York. - 1940-6045 .- 0893-2336. ; 130, s. 327-351
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http://dx.doi.org/10...
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Abstract
Subject headings
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- Direct electrochemical measurements of glutamate release in vivo were combined with optogenetics in order to examine light-induced control of glutamate neurotransmission in the rodent brain. Self-referenced recordings of glutamate using ceramic-based microelectrode arrays (MEAs) in hippocampus and frontal cortex demonstrated precise optical control of light-induced glutamate release through channelrhodopsin (ChR2) expression in both rat hippocampus and frontal cortex. Although the virus was only injected unilaterally, bilateral and rostro-caudal expression was observed in slice imaging, indicating diffusion and active transport of the viral particles. Methodology for the optogenetic control of glutamate signaling in the rat brain is thoroughly explained with special attention paid to MEA enzyme coating and cleaning for the benefit of other investigators. These data support that optogenetic control of glutamate signaling is robust with certain advantages as compared to other methods to modulate the in vivo control of glutamate signaling.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Keyword
- Amperometry
- Array
- Biosensor
- Electrochemistry
- Glutamate
- Glutamate oxidase
- Microelectrode
- Neurotransmitter
- Optogenetics
Publication and Content Type
- kap (subject category)
- ref (subject category)
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- By the author/editor
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Burmeister, Jaso ...
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Pomerleau, Franc ...
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Quintero, Jorge ...
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Huettl, Peter
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Ai, Yi
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Jakobsson, Johan
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show more...
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Lundblad, Martin
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Heuer, Andreas
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Slevin, John T
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Gerhardt, Greg A ...
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Neurosciences
- Articles in the publication
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Neuromethods
- By the university
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Lund University