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Search: onr:"swepub:oai:lup.lub.lu.se:02ed7523-6b6b-4e31-90b1-254e27d3901e" > Insulin induces Thr...

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  • Säll, JohannaLund University,Lunds universitet,Proteinfosforylering,Forskargrupper vid Lunds universitet,Protein Phosphorylation,Lund University Research Groups (author)

Insulin induces Thr484 phosphorylation and stabilization of SIK2 in adipocytes

  • Article/chapterEnglish2019

Publisher, publication year, extent ...

  • Elsevier BV,2019

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  • LIBRIS-ID:oai:lup.lub.lu.se:02ed7523-6b6b-4e31-90b1-254e27d3901e
  • https://lup.lub.lu.se/record/02ed7523-6b6b-4e31-90b1-254e27d3901eURI
  • https://doi.org/10.1016/j.cellsig.2018.12.011DOI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:140202300URI

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  • Language:English
  • Summary in:English

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  • Subject category:art swepub-publicationtype
  • Subject category:ref swepub-contenttype

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  • AIMS/HYPOTHESIS: Salt-inducible kinase 2 (SIK2) is downregulated in adipose tissue from obese or insulin-resistant individuals and inhibition of SIK isoforms results in reduced glucose uptake and insulin signalling in adipocytes. However, the regulation of SIK2 itself in response to insulin in adipocytes has not been studied in detail. The aim of our work was to investigate effects of insulin on various aspects of SIK2 function in adipocytes.METHODS: Primary adipocytes were isolated from human subcutaneous and rat epididymal adipose tissue. Insulin-induced phosphorylation of SIK2 and HDAC4 was analyzed using phosphospecific antibodies and changes in the catalytic activity of SIK2 with in vitro kinase assay. SIK2 protein levels were analyzed in primary adipocytes treated with the proteasome inhibitor MG132.RESULTS: We have identified a novel regulatory pathway of SIK2 in adipocytes, which involves insulin-induced phosphorylation at Thr484. This phosphorylation is impaired in individuals with a reduced insulin action. Insulin stimulation does not affect SIK2 catalytic activity or cellular activity towards HDAC4, but is associated with increased SIK2 protein levels in adipocytes.CONCLUSION/INTERPRETATION: Our data suggest that downregulation of SIK2 in the adipose tissue of insulin-resistant individuals can partially be caused by impaired insulin signalling, which might result in defects in SIK2 expression and function.

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  • Negoita, FlorentinaLund University,Lunds universitet,Molekylär endokrinologi,Forskargrupper vid Lunds universitet,Proteinfosforylering,Molecular Endocrinology,Lund University Research Groups,Protein Phosphorylation(Swepub:lu)med-flf (author)
  • Hansson, BjörnLund University,Lunds universitet,Glukostransport och proteintrafficking,Forskargrupper vid Lunds universitet,Glucose Transport and Protein Trafficking,Lund University Research Groups(Swepub:lu)med-bnh (author)
  • Kopietz, FranziskaLund University,Lunds universitet,Proteinfosforylering,Forskargrupper vid Lunds universitet,Protein Phosphorylation,Lund University Research Groups(Swepub:lu)med-fkz (author)
  • Linder, WilhelmLund University (author)
  • Pettersson, Annie M LKarolinska Institute (author)
  • Ekelund, MikaelSkåne University Hospital(Swepub:lu)mphy-mek (author)
  • Laurencikiene, JurgaKarolinska Institutet,Karolinska Institute (author)
  • Degerman, EvaLund University,Lunds universitet,Signaltransduktionsforskning,Forskargrupper vid Lunds universitet,Insulin Signal Transduction,Lund University Research Groups(Swepub:lu)medk-ede (author)
  • Stenkula, Karin GLund University,Lunds universitet,Glukostransport och proteintrafficking,Forskargrupper vid Lunds universitet,Glucose Transport and Protein Trafficking,Lund University Research Groups(Swepub:lu)med-ksu (author)
  • Göransson, OlgaLund University,Lunds universitet,Proteinfosforylering,Forskargrupper vid Lunds universitet,Protein Phosphorylation,Lund University Research Groups(Swepub:lu)medk-ogo (author)
  • ProteinfosforyleringForskargrupper vid Lunds universitet (creator_code:org_t)

Related titles

  • In:Cellular Signalling: Elsevier BV55, s. 73-801873-39130898-6568

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