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Bacterial identification by optical mapping of genomic DNA in nanofluidic channels

Nyblom, My, 1995 (author)
Chalmers University of Technology,Chalmers tekniska högskola
Müller, Vilhelm, 1990 (author)
Chalmers University of Technology,Chalmers tekniska högskola
Johnning, Anna, 1985 (author)
University of Gothenburg,Chalmers University of Technology,Chalmers tekniska högskola,Göteborgs universitet,Stiftelsen Fraunhofer-Chalmers Centrum för Industrimatematik (FCC),Fraunhofer-Chalmers Research Centre for Industrial Mathematics (FCC)
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Wrande, Marie (author)
Uppsala University,Uppsala universitet
Dvirnas, Albertas (author)
Lund University,Lunds universitet,Beräkningsbiologi och biologisk fysik - Genomgår omorganisation,Institutionen för astronomi och teoretisk fysik - Genomgår omorganisation,Naturvetenskapliga fakulteten,Computational Biology and Biological Physics - Undergoing reorganization,Department of Astronomy and Theoretical Physics - Undergoing reorganization,Faculty of Science
Kesarimangalam, Sriram, 1983 (author)
Chalmers University of Technology,Chalmers tekniska högskola
Giske, Christian G. (author)
Karolinska Institutet,Karolinska University Hospital,Karolinska universitetssjukhuset
Ambjörnsson, Tobias (author)
Lund University,Lunds universitet,Beräkningsbiologi och biologisk fysik - Genomgår omorganisation,Institutionen för astronomi och teoretisk fysik - Genomgår omorganisation,Naturvetenskapliga fakulteten,Computational Biology and Biological Physics - Undergoing reorganization,Department of Astronomy and Theoretical Physics - Undergoing reorganization,Faculty of Science
Sandegren, Linus (author)
Uppsala University,Uppsala universitet
Kristiansson, Erik, 1978 (author)
University of Gothenburg,Chalmers University of Technology,Göteborgs universitet,Chalmers tekniska högskola
Westerlund, Fredrik, 1978 (author)
Chalmers University of Technology,Chalmers tekniska högskola
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 (creator_code:org_t)
2019
2019
English 2 s.
In: 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. - 9781733419000 ; , s. 821-822
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  • Conference paper (peer-reviewed)
Abstract Subject headings
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  • A variety of pathogenic bacteria can infect humans and the increase in bacteria resistant to common antibiotics is a large threat to human health worldwide. This work presents a method, based on optical DNA mapping (ODM) in nanofluidic channels, that can detect the type of bacterial present in a sample by matching the obtained maps of large DNA molecules to a database of fully assembled bacterial genomes. The extraction and labelling protocol has been designed to work for both Gram-positive and Gram-negative bacteria, not requiring any prior knowledge about the sample content.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Infectious Medicine (hsv//eng)
NATURVETENSKAP  -- Biologi -- Biofysik (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Biophysics (hsv//eng)
NATURVETENSKAP  -- Fysik -- Annan fysik (hsv//swe)
NATURAL SCIENCES  -- Physical Sciences -- Other Physics Topics (hsv//eng)
NATURVETENSKAP  -- Biologi -- Mikrobiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Microbiology (hsv//eng)

Keyword

Bacterial Identification
Nanofluidics
Optical DNA Mapping
Nanofluidics

Publication and Content Type

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